AM1818b

antibody from Abgent

Targeting: BECN1 ATG6, VPS30

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AM1818b

Provider

Abgent

Proper citation

Abgent Cat#AM1818b, RRID:AB_10614044

Product name

BECN1 Antibody (Ascites)

Antibody type

Monoclonal

Antigen

Recombinant Protein

Description

Purified Mouse Monoclonal Antibody (Mab)

Reactivity

Human

Host

Mouse

Isotype

IgM

Vial size

100 µl

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Western blot analysis of anti-BECN1 Monoclonal Antibody (Cat. #AM1818b) in Jurkat cell line lysates (35ug/lane). BECN1 (arrow) was detected using the Mab ascites (1:100 dilution).

Primary Ab dilution

1:100~400

Immunocytochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Immunofluorescence analysis of BECN1 Monoclonal Antibody with paraffin-embedded human brain tissue .0.05 mg/ml primary antibody was followed by PE-conjugated goat anti-mouse lgG (whole molecule). PE emits orange fluorescence.

Primary Ab dilution

1:10~50

Immunohistochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"Formalin-fixed and paraffin-embedded human brain with BECN1 Monoclonal Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated."

Primary Ab dilution

1:50~100