MAB5295

antibody from Novus Biologicals

Targeting: BECN1 ATG6, VPS30

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB5295

Provider

Novus Biologicals

Product name

Mouse Monoclonal Beclin 1 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human Beclin 1 in direct ELISAs. Detects human, mouse, and rat Beclin 1 in Western blots. In direct ELISAs, no cross-reactivity with recombinant human ATG3, 4A, 4B, 5, 7, 10, 12, or 16L1 is observed.

Reactivity

Human, Mouse, Rat

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human, Mouse, and Rat Beclin 1 by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line, A20 mouse B cell lymphoma cell line, and NRK rat normal kidney cell line. PVDF Membrane was probed with 0.1 µg/mL of Mouse Anti-Human Beclin 1 Monoclonal Antibody (Catalog # MAB5295) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Beclin 1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human Beclin 1 by Simple Western^TM. Simple Western lane view shows lysates of human brain (cerebellum) tissue, loaded at 0.2 mg/mL. A specific band was detected for Beclin 1 at approximately 58 kDa (as indicated) using 1 µg/mL of Mouse Anti-Human/Mouse/Rat Beclin 1 Monoclonal Antibody (Catalog # MAB5295). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Mouse Beclin 1 by Simple Western^TM. Simple Western lane view shows lysates of mouse brain tissue, loaded at 0.2 mg/mL. A specific band was detected for Beclin 1 at approximately 58 kDa (as indicated) using 1 µg/mL of Mouse Anti-Human/Mouse/Rat Beclin 1 Monoclonal Antibody (Catalog # MAB5295). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.