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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- AP1818a - Provider product page
- Provider
- Abcepta
- Proper citation
- Abgent Cat#AP1818a, RRID:AB_2064336
- Product name
- Beclin 1 Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 2 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Submitted references Expression of LC3 and Beclin 1 in the spinal dorsal horn following spinal nerve ligation-induced neuropathic pain.
Triterpenes from Ganoderma Lucidum induce autophagy in colon cancer through the inhibition of p38 mitogen-activated kinase (p38 MAPK).
Chondrocyte autophagy is stimulated by HIF-1 dependent AMPK activation and mTOR suppression.
Zhang E, Yi MH, Ko Y, Kim HW, Seo JH, Lee YH, Lee W, Kim DW
Brain research 2013 Jun 26;1519:31-9
Brain research 2013 Jun 26;1519:31-9
Triterpenes from Ganoderma Lucidum induce autophagy in colon cancer through the inhibition of p38 mitogen-activated kinase (p38 MAPK).
Thyagarajan A, Jedinak A, Nguyen H, Terry C, Baldridge LA, Jiang J, Sliva D
Nutrition and cancer 2010;62(5):630-40
Nutrition and cancer 2010;62(5):630-40
Chondrocyte autophagy is stimulated by HIF-1 dependent AMPK activation and mTOR suppression.
Bohensky J, Leshinsky S, Srinivas V, Shapiro IM
Pediatric nephrology (Berlin, Germany) 2010 Apr;25(4):633-42
Pediatric nephrology (Berlin, Germany) 2010 Apr;25(4):633-42
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Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- Western blot analysis of anti-hBECN1-Q196 Pab (Cat. #AP1818a) in 293 cell line lysates transiently transfected with the BECN1 gene (2ug/lane). hBECN1-Q196(arrow) was detected using the purified Pab.
- Primary Ab dilution
- 1:1000
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- The anti-BECN1 Pab (Cat. #AP1818a) is used in Western blot to detect BECN1 in mouse liver tissue lysate. BECN1(arrow) was detected using the purified Pab.
- Primary Ab dilution
- 1:1000
Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- "Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma."
- Primary Ab dilution
- 1:50~100
