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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- PA5-35394 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-Beclin 1 (Ser295) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with bovine, canine, chicken, mouse, non-human primate, sheep, Xenopus and zebrafish based on 100% sequence homology. In Western blot, additional bands may be observed when using non-immunoprecipitated lysates.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.5 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot of Beclin 1 in wild type beclin-1 expressed in HeLa cell lysate showing specific immunolabeling of Phospho-Beclin 1 (Ser295) polyclonal antibody (Product # PA5-35394). This immunolabeling is eliminated in the beclin-1 alanine substitution mutant (S295A).
