AM20210PU-N

antibody from Acris Antibodies GmbH

Targeting: BECN1 ATG6, VPS30

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AM20210PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AM20210PU-N, RRID:AB_10758864

Product name

anti Beclin-1

Antibody type

Monoclonal

Antigen

Synthetic peptide conjugated to hemocyanin derived from Beclin sequence.

Reactivity

Human, Mouse, Rat, Canine

Host

Mouse

Isotype

IgG

Antibody clone number

12B4

Vial size

0.1 mg

Concentration

0.1 mg/ml

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Detection of endogenous Beclin: Whole cell lysates of untreated tumor cells were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with AM20210PU-N Beclin antibody (Clone 12B4) at 0.5 µg/ ml for 1h at RT and developed by ECL (exp. time: 30 sec).Lane 1: HeLaLane 2: HepG2 Lane 3: HEK 293 Lane 4: SH-SY5Y Lane 5: MDCK Lane 6: PC12Lane 7: CMT Lane 8: Neuro2A