Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- AM20210PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AM20210PU-N, RRID:AB_10758864
- Product name
- anti Beclin-1
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide conjugated to hemocyanin derived from Beclin sequence.
- Reactivity
- Human, Mouse, Rat, Canine
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 12B4
- Vial size
- 0.1 mg
- Concentration
- 0.1 mg/ml
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Supportive validation
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- Acris Antibodies GmbH (provider)
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- Experimental details
- Detection of endogenous Beclin: Whole cell lysates of untreated tumor cells were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with AM20210PU-N Beclin antibody (Clone 12B4) at 0.5 µg/ ml for 1h at RT and developed by ECL (exp. time: 30 sec).Lane 1: HeLaLane 2: HepG2 Lane 3: HEK 293 Lane 4: SH-SY5Y Lane 5: MDCK Lane 6: PC12Lane 7: CMT Lane 8: Neuro2A