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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [4]
- Flow cytometry [1]
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- Product number
- R31504 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Beclin 1 Antibody
- Antibody type
- Polyclonal
- Antigen
- Human partial recombinant protein (AA 1-354) was used as the immunogen for this Beclin 1 antibody.
- Description
- Antigen affinity purified antibody
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 µg
- Concentration
- Lyophilized; resuspend with 200 ul for 0.5 mg/ml
- Storage
- After reconstitution, the Beclin 1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of Beclin 1 antibody and Lane 1: COLO320; 2: HepG2; 3: PANC; 4: A431; 5: SMMC-7721; 6: Jurkat; 7: SW620; 8: U87 lysate. Expected size: 49KD, Observed size: ~57KD
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human Raji, 2) human 293T, 3) human MCF7, 4) human PC-3, 5) rat C6, 6) rat PC-12, 7) mouse NIH 3T3 and 8) mouse RAW264.7 cell lysate with Beclin 1 antibody. Predicted molecular weight ~52 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human 293T, 2) human MCF7, 3) human PC-3, 4) rat C6, 5) rat PC-12, 6) mouse NIH 3T3 and 7) mouse RAW264.7 cell lysate with Beclin 1 antibody and DyLight 647 secondary. Predicted molecular weight ~52 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE human HeLa cells with Beclin 1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunoprecipitation of Beclin 1 protein from 500ug of human MCF7 whole cell lysate with 2ug of Beclin 1 antibody.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P: Beclin 1 antibody testing of mouse spleen tissue
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P testing of mouse intestine tissue
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P testing of rat spleen tissue
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P testing of rat intestine tissue
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of fixed and permeabilized human Raji cells with Beclin 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Beclin 1 antibody.
