AM20210PU-N

antibody from OriGene

Targeting: BECN1 ATG6, VPS30

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AM20210PU-N

Provider

OriGene

Product name

Beclin 1 (BECN1) mouse monoclonal antibody, clone 12B4, Purified

Antibody type

Monoclonal

Description

Beclin 1 (BECN1) mouse monoclonal antibody, clone 12B4, Purified

Host

Mouse

Conjugate

Unconjugated

Epitope

BECN1

Isotype

IgG

Antibody clone number

12B4

Vial size

100 µg

Concentration

0.1 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Detection of endogenous Beclin: Whole cell lysates of untreated tumor cells were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with Beclin antibody (Clone 12B4) at 0.5 ug/ ml for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: HeLa Lane 2: HepG2 Lane 3: HEK 293 Lane 4: SH-SY5Y Lane 5: MDCK Lane 6: PC12 Lane 7: CMT Lane 8: Neuro2A

Validation comment

WB