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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
- Flow cytometry [1]
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- Product number
- TA502517 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA502517, RRID:AB_11127032
- Product name
- BECN1 (Beclin 1) mouse monoclonal antibody, clone OTI3H8 (formerly 3H8)
- Antibody type
- Monoclonal
- Description
- BECN1 (Beclin 1) mouse monoclonal antibody, clone OTI3H8 (formerly 3H8)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- BECN1
- Isotype
- IgG
- Antibody clone number
- OTI3H8
- Vial size
- 100 µl
- Concentration
- 0.55 mg/ml
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BECN1 (RC201629, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BECN1.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BECN1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-BECN1 mouse monoclonal antibody (TA502517) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BECN1(RC201629).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-BECN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502517)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-BECN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502517)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-BECN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502517)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC201629 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-BECN1 antibody(TA502517), and then analyzed by flow cytometry.
- Validation comment
- FC
