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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- TA502527 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA502527, RRID:AB_11126239
- Product name
- BECN1 (Beclin 1) mouse monoclonal antibody, clone OTI3F3 (formerly 3F3)
- Antibody type
- Monoclonal
- Description
- BECN1 (Beclin 1) mouse monoclonal antibody, clone OTI3F3 (formerly 3F3)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- BECN1
- Isotype
- IgG
- Antibody clone number
- OTI3F3
- Vial size
- 100 µl
- Concentration
- 0.78 mg/ml
Submitted references Regulation of SREBP-2 intracellular trafficking improves impaired autophagic flux and alleviates endoplasmic reticulum stress in NAFLD.
Deng X, Pan X, Cheng C, Liu B, Zhang H, Zhang Y, Xu K
Biochimica et biophysica acta. Molecular and cell biology of lipids 2017 Mar;1862(3):337-350
Biochimica et biophysica acta. Molecular and cell biology of lipids 2017 Mar;1862(3):337-350
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BECN1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BECN1 (RC201629, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BECN1.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-BECN1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-BECN1 mouse monoclonal antibody (TA502527) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BECN1(RC201629).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-BECN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502527)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC201629 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-BECN1 antibody(TA502527), and then analyzed by flow cytometry.
- Validation comment
- FC
