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- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- MA5-15825 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Beclin 1 Monoclonal Antibody (2A4)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-15825 targets BECN1 in indirect ELISA, FACS, IHC, and WB applications and shows reactivity with Human samples. The MA5-15825 immunogen is purified recombinant fragment of human BECN1 expressed in E. Coli. MA5-15825 detects BECN1 which has a predicted molecular weight of approximately 60kDa.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2A4
- Vial size
- 100 µL
- Concentration
- Conc. Not Determined
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Lipoxin A4 promotes autophagy and inhibits overactivation of macrophage inflammasome activity induced by Pg LPS.
Mechanistic studies of cytotoxic activity of the mesoionic compound MIH 2.4Bl in MCF-7 breast cancer cells.
Gambogic acid inhibits proliferation and induces apoptosis of human acute T‑cell leukemia cells by inducing autophagy and downregulating β‑catenin signaling pathway: Mechanisms underlying the effect of Gambogic acid on T‑ALL cells.
Zhao J, Geng W, Wan K, Guo K, Xi F, Xu X, Xiong X, Huang X, Liu J, Kuang X
The Journal of international medical research 2021 Feb;49(2):300060520981259
The Journal of international medical research 2021 Feb;49(2):300060520981259
Mechanistic studies of cytotoxic activity of the mesoionic compound MIH 2.4Bl in MCF-7 breast cancer cells.
de Mascena Costa LA, Debnath D, Harmon AC, de Sousa Araújo S, da Silva Souza HD, de Athayde Filho PF, Wischral A, Adrião Gomes Filho M, Mathis JM
Oncology letters 2020 Sep;20(3):2291-2301
Oncology letters 2020 Sep;20(3):2291-2301
Gambogic acid inhibits proliferation and induces apoptosis of human acute T‑cell leukemia cells by inducing autophagy and downregulating β‑catenin signaling pathway: Mechanisms underlying the effect of Gambogic acid on T‑ALL cells.
Wang T, Du J, Kong D, Yang G, Zhou Q, You F, Lin Y, Wang Y
Oncology reports 2020 Oct;44(4):1747-1757
Oncology reports 2020 Oct;44(4):1747-1757
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of A549 (Lane 1), SH-SY5Y (Lane 2), HeLa (Lane 3), MKN45 (Lane 4), Raji (Lane 5), Jurkat (Lane 6), KARPAS 299 (Lane 7) and SW480 (Lane 8). The blot was probed with Anti-Beclin 1 Monoclonal Antibody (2A4) (Product # MA5-15825, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 52 kDa band corresponding to Beclin 1 was observed across the cell lines tested.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of BECN1 using BECN1 monoclonal antibody (Product # MA5-15825) in HeLa (1), A431 (2), MCF-7 (3), RAJI (4), Jurkat (5) and SKBR-3 (6) cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of Beclin 1 was achieved by transfecting HeLa with Beclin 1 specific siRNAs (Silencer® select Product # s16537, s16539). Western blot analysis (Fig. a) was performed using whole cell extracts from the HeLa knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with Beclin 1 Monoclonal Antibody (2A4) (Product # MA5-15825, 1:500 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Reduction of signal upon siRNA mediated knock down confirms that antibody is specific to Beclin 1.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded breast cancer tissues (left) and liver cancer tissues (right) using BECN1 monoclonal antibody (Product # MA5-15825) followed with DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of RAJI cells using BECN1 monoclonal antibody (Product # MA5-15825) (green) and negative control (purple).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2. Effect of MIH 2.4Bl on the induction of autophagy in MCF-7 cells. (A) Expression of Beclin-1, ATG5, LC3B II/I and GAPDH were determined by western blot analysis in control or MIH 2.4Bl treated cells after 48 or 72 h of culture. (B) Densitometry analysis of Beclin-1, ATG5, LC3B II and LC3B I expression was performed and normalized to GAPDH. Data are presented as histograms of normalized Beclin-1 and ATG5 intensity values, and the intensity ratios of LC3B-II to LC3B-I. LC3B, microtubule-associated protein 1A/1B light chain 3B. C, control cells; M, MIH 2.4Bl treated cells.
