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Learnsc-48385
antibody from Santa Cruz Biotechnology
Targeting: POLR1A
DKFZP586M0122, FLJ21915, RPA1, RPA190, RPO1-4
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- sc-48385 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-48385, RRID:AB_675814
- Product name
- Anti-POLR1A
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Mouse
Submitted references Computer-based fluorescence quantification: a novel approach to study nucleolar biology.
Kodiha M, Bański P, Stochaj U
BMC cell biology 2011 Jun 3;12:25
BMC cell biology 2011 Jun 3;12:25
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Supportive validation
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- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Band of predicted size in kDa (+/-20%) with additional bands present.
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:7000
- Lane 1
- Marker [kDa]: 219, 111, 83, 48, 32, 26, 17
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 48
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human stomach shows strong nuclear positivity in glandular cells.
- Validation comment
- Staining pattern consistent with experimental and/or bioinformatic data.
