NB300-144

antibody from Novus Biologicals

Targeting: LAMA1 LAMA

Immunocytochemistry

Antibody data

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Validation data

Product number: NB300-144 - Provider product page
Provider: Novus Biologicals
Proper citation: Novus Cat#NB300-144, RRID:AB_10001146
Product name: Rabbit Polyclonal Laminin Antibody
Antibody type: Polyclonal
Description: IgG purified. Laminin Antibody is pan-specific and reacts well with all Laminin isoforms tested: Laminin-1 (alpha-1, beta-1, and gamma-1) and Laminin-2 (alpha-2, beta-1, and gamma-1).
Reactivity: Human, Mouse, Rat, Rabbit, Sheep
Host: Rabbit
Isotype: IgG
Vial size: 0.1 ml
Concentration: 1 mg/ml
Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

LAMA1 protein structure - NB300-144 shown in red.

Supportive validation

Submitted by: Novus Biologicals (provider)
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Experimental details: Western Blot: Laminin Antibody [NB300-144] - Analysis of Laminin-1 expression in mouse EHS tumor crude extracts (left) and Laminin-2 expression in rat heart crude extracts (right), using Laminin Antibody [NB300-144]. The Laminin polyclonal antibody was used at 1 ug/mL. Theoretical Molecular Weight of NB300-144 is 337 kDa.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: Laminin Antibody [NB300-144] - Analysis of rat heart cells lysates (lane 1) and 0.2 ug of purified laminin protein from mouse EHS sarcoma (lane 2) Laminin Antibody [NB300-144] recognizes 3 laminin isotypes: alpha 1 (440 kDa), beta 1 (220 kDa) and gamma 1 (220 kDa). Also recognized is a laminin binding protein at 120 kDa in both rat heart lysates and purified laminin protein. Since this protein always coexpresses with laminin this crossreactivity is irrelevant. Theoretical molecular weight of LAMA1 is 337 kDa.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: Laminin Antibody [NB300-144] - Analysis of different tissue lysates using rabbit pAb to laminin, NB300-144, dilution 1:5,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, and [4] cow spinal cord lysate. The strong band above the 280kDa mark corresponds to full length laminin proteins. Smaller proteolytic fragments of laminin are also detected with this antibody.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: Laminin Antibody [NB300-144] - Western blot analysis of the effects of IP6, Ins, IP6 + Ins and normal saline on the levels of collagen IV, Laminin and Fibronectin, with Laminin Antibody [NB300-144]. IP6 or Ins treatment decreased the protein expression of LN and FN, and the combined IP6 + Ins treatment resulted in significantly greater effects compared with treatment with either compound alone. The Western blot membranes were stripped and reprobed for beta-actin as an internal control to confirm equal loading. (A) representative blots from one of three separate experiments; (B) relative band intensities based on densitometry. The results are expressed as the mean +/- standard deviation from three independent experiments. * p < 0.05 compared to the IP6 + Ins group; #p < 0.05 compared to the normal saline group. Image collected and cropped by CiteAb from the following publication (http://www.mdpi.com/2072-6643/8/5/286), licensed under a CC-BY licence.

Supportive validation

Submitted by: Novus Biologicals (provider)
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Experimental details: Immunohistochemistry: Laminin Antibody [NB300-144] - Staining of rat spinal cord and dorsal root paraformaldehyde/paraffin-embedded tissue using Laminin Antibody [NB300-144]. Pepsin antigen retrieval was performed on this tissue sample.

Supportive validation

Submitted by: Novus Biologicals (provider)
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Experimental details: Immunohistochemistry: Laminin Antibody [NB300-144] - MxIF analysis of mouse submandibular salivary gland morphogenesis. MxIF of a developmental TMA including embryonic stages (E14, E16, E18) and postnatal stages (P5 and P20) was performed using sequential application of directly conjugated antibodies to detect multiple markers of tissue structures and cell types on the same tissue sections. Tissue compartments. The epithelium, mesenchyme, neurons, and basement membranes was detected using antibodies directed towards E-cadherin (ECAD, red), platelet-derived growth factor (PDGFR, green), beta III tubulin (bIII, magenta), and Laminin Antibody [NB300-144] (LMN, cyan), respectively. Image collected and cropped by CiteAb from the following publication (http://bio.biologists.org/cgi/doi/10.1242/bio.20134309), licensed under a CC-BY licence.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry: Laminin Antibody [NB300-144] - VSOP observed in perivascular-restricted spinal cord lesions with intact BBB. Immunostaining for laminin (brown) using Laminin Antibody [NB300-144] shows vascular endothelium and glia limitans of a perivascular lesion, along with infiltrating cells and VSOP (blue). Image collected and cropped by CiteAb from the following publication (http://asn.sagepub.com/lookup/doi/10.1042/AN20120081), licensed under a CC-BY licence.