H00027032-M01
antibody from Novus Biologicals
Targeting: ATP2C1
ATP2C1A, BCPM, KIAA1347, PMR1, SPCA1
Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [3]
- ELISA [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- H00027032-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00027032-M01, RRID:AB_537005
- Product name
- Mouse Monoclonal ATP2C1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. ATP2C1 - ATPase, Ca++ transporting, type 2C, member 1
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Activity of the SPCA1 Calcium Pump Couples Sphingomyelin Synthesis to Sorting of Secretory Proteins in the Trans-Golgi Network.
Diverse Viruses Require the Calcium Transporter SPCA1 for Maturation and Spread.
Cofilin recruits F-actin to SPCA1 and promotes Ca2+-mediated secretory cargo sorting.
Golgi calcium pump secretory pathway calcium ATPase 1 (SPCA1) is a key regulator of insulin-like growth factor receptor (IGF1R) processing in the basal-like breast cancer cell line MDA-MB-231.
Deng Y, Pakdel M, Blank B, Sundberg EL, Burd CG, von Blume J
Developmental cell 2018 Nov 19;47(4):464-478.e8
Developmental cell 2018 Nov 19;47(4):464-478.e8
Diverse Viruses Require the Calcium Transporter SPCA1 for Maturation and Spread.
Hoffmann HH, Schneider WM, Blomen VA, Scull MA, Hovnanian A, Brummelkamp TR, Rice CM
Cell host & microbe 2017 Oct 11;22(4):460-470.e5
Cell host & microbe 2017 Oct 11;22(4):460-470.e5
Cofilin recruits F-actin to SPCA1 and promotes Ca2+-mediated secretory cargo sorting.
Kienzle C, Basnet N, Crevenna AH, Beck G, Habermann B, Mizuno N, von Blume J
The Journal of cell biology 2014 Sep 1;206(5):635-54
The Journal of cell biology 2014 Sep 1;206(5):635-54
Golgi calcium pump secretory pathway calcium ATPase 1 (SPCA1) is a key regulator of insulin-like growth factor receptor (IGF1R) processing in the basal-like breast cancer cell line MDA-MB-231.
Grice DM, Vetter I, Faddy HM, Kenny PA, Roberts-Thomson SJ, Monteith GR
The Journal of biological chemistry 2010 Nov 26;285(48):37458-66
The Journal of biological chemistry 2010 Nov 26;285(48):37458-66
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ATP2C1 Antibody (2G1) [H00027032-M01] - Analysis of ATP2C1 over-expressed 293 cell line, cotransfected with ATP2C1 Validated Chimera RNAi ( Cat # H00027032-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ATP2C1 monoclonal antibody (M01), clone 2G1 (Cat # H00027032-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ATP2C1 Antibody (2G1) [H00027032-M01] - Analysis of ATP2C1 expression in transfected 293T cell line by ATP2C1 monoclonal antibody (M01), clone 2G1.Lane 1: ATP2C1 transfected lysate(100.6 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ATP2C1 Antibody (2G1) [H00027032-M01] - ATP2C1 monoclonal antibody (M01), clone 2G1 Analysis of ATP2C1 expression in HeLa.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: ATP2C1 Antibody (2G1) [H00027032-M01] - Detection limit for recombinant GST tagged ATP2C1 is approximately 0.03ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: ATP2C1 Antibody (2G1) [H00027032-M01] - Analysis of monoclonal antibody to ATP2C1 on HeLa cell. Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: ATP2C1 Antibody (2G1) [H00027032-M01] - Analysis of ATP2C1 transfected lysate using anti-ATP2C1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ATP2C1 MaxPab rabbit polyclonal antibody.