Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- LS-B2830 - Provider product page
- Provider
- LSBio
- Proper citation
- LifeSpan Cat#LS-B2830, RRID:AB_2264842
- Product name
- IHC-plus™ IL7R / CD127 Antibody LS-B2830
- Antibody type
- Polyclonal
- Description
- Antigen affinity chromatography
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.
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Supportive validation
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- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Dot Blot of Rabbit anti-IL-7 Receptor antibody. Antigen: IL-7 Receptor and IL-7 Receptor pY449 forms of the immunizing peptide and THRB1 and THRB1 pY171 as controls. Load: 3.0, 0.3, 0.1, 0.05 ug as indicated. Primary antibody: IL-7 Receptor antibody at 1:400 for 45 min at 4°C. Secondary antibody: Dylight488 rabbit secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C.