Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- LS-B2831 - Provider product page
- Provider
- LSBio
- Proper citation
- LifeSpan Cat#LS-B2831, RRID:AB_2126107
- Product name
- IHC-plus™ IL7R / CD127 Antibody (phospho-Tyr449) LS-B2831
- Antibody type
- Polyclonal
- Description
- Affinity purified
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
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Supportive validation
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- LSBio (provider)
- Enhanced method
- Genetic validation
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- Experimental details
- Anti-IL-7 Receptor alpha chain pY449 - Western Blot. Anti-IL-7 Receptor alpha chain pY449 in immunoprecipitation and Western blot. Lysates were prepared from thymocyte D1 cells treated with or without IL7 (50 ng/ml), and immunoprecipitated with anti-Phosphotyrosine conjugated to Protein G agarose. After washing and boiling, the supernatant was separated by SDS-PAGE and transferred to nitrocellulose. Western Blot analysis was performed using anti-IL7 receptor pY449, diluted 1:10000 in BSA, incubated overnight at 4°C. The proteins were visualized using an HRP-conjugated goat anti-rabbit antibody and ECL detection.