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Western blot
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- MAB306-100 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal IL-7R alpha/CD127 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects human IL-7 R alpha in Western blots. In Western blots, approximately 5% cross-reactivity was observed with recombinant human (rh) IL-10 R, rhIL-2 R beta , rhIL-5 R alpha , and rhIL-6 R.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of IL-7 R alpha/CD127 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3 epsilon PE-conjugated Monoclonal Antibody (Catalog # FAB100P) and either (A) Mouse Anti-Human IL-7 R alpha/CD127 Monoclonal Antibody (Catalog # MAB306) or (B) Mouse IgG1 Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).
