351344

antibody from BioLegend

Targeting: IL7R CD127, IL7RA

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

351344

Provider

BioLegend

Proper citation

BioLegend Cat#351344, RRID:AB_2566200

Product name

Alexa Fluor® 700 anti-human CD127 (IL-7Rα)

Antibody type

Monoclonal

Antigen

Recombinant human CD127

Description

CD127 is a 60-90 kD type I transmembrane glycoprotein also known as IL-7 receptor α chain or IL-7Rα. It forms a heterodimer with the common γ chain (γc or CD132) which is shared with the receptors for IL-2, IL-4, IL-9, IL-13, IL-15, and IL-21. CD127 is expressed on immature B cells through early pre-B stage cells, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, and bone marrow stromal cells. CD127 has been reported to be a useful marker for identifying memory and effector T cells. Studies have shown that CD127 expression is down-modulated on Treg cells. It can be used as a marker for differentiation of Treg and conventional T cells. The ligation of IL-7 with its receptor is important for stimulation of mature and immature T cells as well as immature B cell proliferation and development.

Reactivity

Human

Host

Mouse

Conjugate

Near infrared dye

Antibody clone number

A019D5

Vial size

100 tests

Storage

2°C-8°C

Handling

Ambient RT

Flow cytometry

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Human peripheral blood lymphocytes were stained with CD3 PE and CD127 (clone A019D5) Alexa Fluor® 700 (top) or mouse IgG1, κ Alexa Fluor® 700 isotype control (bottom).

Conjugate

Near infrared dye