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- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- TA319456 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal IL-7 Receptor Alpha Chain phospho Y449 antibody (Phospho-specific)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal IL-7 Receptor Alpha Chain phospho Y449 antibody (Phospho-specific)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- Il7r
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µg
- Concentration
- 0.82 mg/mL
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- WB and IP of Rabbit anti- IL-7-Receptor-alpha-chain-pY449 antibody. Lane 1: thymocyte D1 cells. Lane 2: thymocyte D1 cells treated with IL7 (50 ng/ml). IP: with anti-Phosphotyrosine conjugated to Protein G agarose. Primary antibody: IL7pY449 antibody at 1:10000 for overnight at 4°C. Secondary antibody: HRP-conjugated goat anti-rabbit antibody at 1:10,000 for 45 min at RT and ECL detection. Block: 5% BLOTTO overnight at 4°C.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western Blot of Rabbit anti- IL-7-Receptor-alpha-chain-pY449 antibody. Load: thymocyte D1 cells treated with or without IL-7 (50 ng/ml) for 20 min. Primary antibody: IL7pY449 antibody at 1:1000 for overnight at 4°C. Secondary antibody: IRDye800? rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 55 kDa, ~64 kDa for IL7pY449. Other band(s): unspecifics.
- Validation comment
- WB
