Antibody data
- Antibody Data
- Antigen structure
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- Product number
- 14-1278-82 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD127 Monoclonal Antibody (eBioRDR5), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The eBioRDR5 monoclonal antibody reacts with human CD127 (Interleukin-7 Receptor alpha). CD127 complexes with CD132, also known as the common gamma chain (gamma c), to form the multi-functional IL-7 receptor (IL-7R). CD127 is a type I glycoprotein with a molecular weight of 75-80 kDa and is expressed by immature B cells through the early pre-B stage, by thymocytes during several stages of their development, and on most mature T cells, with transient down-regulation upon activation. Binding of IL-7 results in signal transduction which occurs through several tyrosine kinase pathways including the Jak/STAT pathway. IL-7 is indispensible for the development of lymphocytes, and the control of homeostatic proliferation of T-cells in the periphery. In addition, IL-7R signaling is know to be involved in the regulation of T cell receptor (TCR) locus rearrangement in gamma delta T cells.
- Antibody clone number
- eBioRDR5
- Concentration
- 0.5 mg/mL
Submitted references Epinephrine Production in Th17 Cells and Experimental Autoimmune Encephalitis.
Immunophenotype of Gastric Tumors Unveils a Pleiotropic Role of Regulatory T Cells in Tumor Development.
Transdifferentiation of tumor infiltrating innate lymphoid cells during progression of colorectal cancer.
The Effects of High Mobility Group Box-1 Protein on Peripheral Treg/Th17 Balance in Patients with Atherosclerosis.
Activation of miR-21-Regulated Pathways in Immune Aging Selects against Signatures Characteristic of Memory T Cells.
A probiotic modulates the microbiome and immunity in multiple sclerosis.
Characterization of CD127(-) CD25(++) Treg from human colostrum.
Differentially Expressed Potassium Channels Are Associated with Function of Human Effector Memory CD8(+) T Cells.
Serial immunomonitoring of cancer patients receiving combined antagonistic anti-CD40 and chemotherapy reveals consistent and cyclical modulation of T cell and dendritic cell parameters.
Analysis of the cross-talk of Epstein-Barr virus-infected B cells with T cells in the marmoset.
HDAC inhibition potentiates immunotherapy in triple negative breast cancer.
Blocking the recruitment of naive CD4(+) T cells reverses immunosuppression in breast cancer.
Blockade of CD127 Exerts a Dichotomous Clinical Effect in Marmoset Experimental Autoimmune Encephalomyelitis.
The Distribution of Human Stem Cell-like Memory T Cell in Lung Cancer.
T-cell activation is an immune correlate of risk in BCG vaccinated infants.
TLR7 induces anergy in human CD4(+) T cells.
Enhanced suppressor function of TIM-3+ FoxP3+ regulatory T cells.
CD20+ B cell depletion alters T cell homing.
Superiority of rapamycin over tacrolimus in preserving nonhuman primate Treg half-life and phenotype after adoptive transfer.
Regulatory T cells exhibit decreased proliferation but enhanced suppression after pulsing with sirolimus.
Significant mobilization of both conventional and regulatory T cells with AMD3100.
CD28 costimulation Impairs the efficacy of a redirected t-cell antitumor attack in the presence of regulatory t cells which can be overcome by preventing Lck activation.
Fc receptor-like 3 protein expressed on IL-2 nonresponsive subset of human regulatory T cells.
IL-7 enhances thymic human T cell development in "human immune system" Rag2-/-IL-2Rgammac-/- mice without affecting peripheral T cell homeostasis.
Allosuppressive donor CD4+CD25+ regulatory T cells detach from the graft and circulate in recipients after liver transplantation.
Loss of IL-7 receptor alpha on CD4+ T cells defines terminally differentiated B cell-helping effector T cells in a B cell-rich lymphoid tissue.
Loss of IL-7 receptor alpha on CD4+ T cells defines terminally differentiated B cell-helping effector T cells in a B cell-rich lymphoid tissue.
CD127 expression inversely correlates with FoxP3 and suppressive function of human CD4+ T reg cells.
Expression of interleukin (IL)-2 and IL-7 receptors discriminates between human regulatory and activated T cells.
The interleukin-7 receptor alpha chain transmits distinct signals for proliferation and differentiation during B lymphopoiesis.
Cloning of the human and murine interleukin-7 receptors: demonstration of a soluble form and homology to a new receptor superfamily.
Yang P, Tian H, Zou YR, Chambon P, Ichinose H, Honig G, Diamond B, Kim SJ
Frontiers in immunology 2021;12:616583
Frontiers in immunology 2021;12:616583
Immunophenotype of Gastric Tumors Unveils a Pleiotropic Role of Regulatory T Cells in Tumor Development.
Rocha S, Basto AP, Ijsselsteijn ME, Teles SP, Azevedo MM, Gonçalves G, Gullo I, Almeida GM, Maqueda JJ, Oliveira MI, Carneiro F, Barata JT, Graça L, de Miranda NFCC, Carvalho J, Oliveira C
Cancers 2021 Jan 23;13(3)
Cancers 2021 Jan 23;13(3)
Transdifferentiation of tumor infiltrating innate lymphoid cells during progression of colorectal cancer.
Wang S, Qu Y, Xia P, Chen Y, Zhu X, Zhang J, Wang G, Tian Y, Ying J, Fan Z
Cell research 2020 Jul;30(7):610-622
Cell research 2020 Jul;30(7):610-622
The Effects of High Mobility Group Box-1 Protein on Peripheral Treg/Th17 Balance in Patients with Atherosclerosis.
Ding JW, Zhou T, Zheng XX, Wang XA, Tong XH, Luo CY, Zhang ZQ, Yu B
Acta Cardiologica Sinica 2018 Sep;34(5):399-408
Acta Cardiologica Sinica 2018 Sep;34(5):399-408
Activation of miR-21-Regulated Pathways in Immune Aging Selects against Signatures Characteristic of Memory T Cells.
Kim C, Hu B, Jadhav RR, Jin J, Zhang H, Cavanagh MM, Akondy RS, Ahmed R, Weyand CM, Goronzy JJ
Cell reports 2018 Nov 20;25(8):2148-2162.e5
Cell reports 2018 Nov 20;25(8):2148-2162.e5
A probiotic modulates the microbiome and immunity in multiple sclerosis.
Tankou SK, Regev K, Healy BC, Tjon E, Laghi L, Cox LM, Kivisäkk P, Pierre IV, Hrishikesh L, Gandhi R, Cook S, Glanz B, Stankiewicz J, Weiner HL
Annals of neurology 2018 Jun;83(6):1147-1161
Annals of neurology 2018 Jun;83(6):1147-1161
Characterization of CD127(-) CD25(++) Treg from human colostrum.
Cérbulo-Vázquez A, Hernández-Peláez G, Arriaga-Pizano LA, Bautista-Pérez P, Romero-Venado J, Flores-González JC, Figueroa-Damian R, Soriano-Becerril D, Mancilla-Herrera I
American journal of reproductive immunology (New York, N.Y. : 1989) 2018 Feb;79(2)
American journal of reproductive immunology (New York, N.Y. : 1989) 2018 Feb;79(2)
Differentially Expressed Potassium Channels Are Associated with Function of Human Effector Memory CD8(+) T Cells.
Sim JH, Kim KS, Park H, Kim KJ, Lin H, Kim TJ, Shin HM, Kim G, Lee DS, Park CW, Lee DH, Kang I, Kim SJ, Cho CH, Doh J, Kim HR
Frontiers in immunology 2017;8:859
Frontiers in immunology 2017;8:859
Serial immunomonitoring of cancer patients receiving combined antagonistic anti-CD40 and chemotherapy reveals consistent and cyclical modulation of T cell and dendritic cell parameters.
McDonnell AM, Cook A, Robinson BWS, Lake RA, Nowak AK
BMC cancer 2017 Jun 15;17(1):417
BMC cancer 2017 Jun 15;17(1):417
Analysis of the cross-talk of Epstein-Barr virus-infected B cells with T cells in the marmoset.
Dunham J, van Driel N, Eggen BJ, Paul C, 't Hart BA, Laman JD, Kap YS
Clinical & translational immunology 2017 Feb;6(2):e127
Clinical & translational immunology 2017 Feb;6(2):e127
HDAC inhibition potentiates immunotherapy in triple negative breast cancer.
Terranova-Barberio M, Thomas S, Ali N, Pawlowska N, Park J, Krings G, Rosenblum MD, Budillon A, Munster PN
Oncotarget 2017 Dec 26;8(69):114156-114172
Oncotarget 2017 Dec 26;8(69):114156-114172
Blocking the recruitment of naive CD4(+) T cells reverses immunosuppression in breast cancer.
Su S, Liao J, Liu J, Huang D, He C, Chen F, Yang L, Wu W, Chen J, Lin L, Zeng Y, Ouyang N, Cui X, Yao H, Su F, Huang JD, Lieberman J, Liu Q, Song E
Cell research 2017 Apr;27(4):461-482
Cell research 2017 Apr;27(4):461-482
Blockade of CD127 Exerts a Dichotomous Clinical Effect in Marmoset Experimental Autoimmune Encephalomyelitis.
Dunham J, Lee LF, van Driel N, Laman JD, Ni I, Zhai W, Tu GH, Lin JC, Bauer J, 't Hart BA, Kap YS
Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology 2016 Mar;11(1):73-83
Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology 2016 Mar;11(1):73-83
The Distribution of Human Stem Cell-like Memory T Cell in Lung Cancer.
Hong H, Gu Y, Sheng SY, Lu CG, Zou JY, Wu CY
Journal of immunotherapy (Hagerstown, Md. : 1997) 2016 Jul-Aug;39(6):233-40
Journal of immunotherapy (Hagerstown, Md. : 1997) 2016 Jul-Aug;39(6):233-40
T-cell activation is an immune correlate of risk in BCG vaccinated infants.
Fletcher HA, Snowden MA, Landry B, Rida W, Satti I, Harris SA, Matsumiya M, Tanner R, O'Shea MK, Dheenadhayalan V, Bogardus L, Stockdale L, Marsay L, Chomka A, Harrington-Kandt R, Manjaly-Thomas ZR, Naranbhai V, Stylianou E, Darboe F, Penn-Nicholson A, Nemes E, Hatherill M, Hussey G, Mahomed H, Tameris M, McClain JB, Evans TG, Hanekom WA, Scriba TJ, McShane H
Nature communications 2016 Apr 12;7:11290
Nature communications 2016 Apr 12;7:11290
TLR7 induces anergy in human CD4(+) T cells.
Dominguez-Villar M, Gautron AS, de Marcken M, Keller MJ, Hafler DA
Nature immunology 2015 Jan;16(1):118-28
Nature immunology 2015 Jan;16(1):118-28
Enhanced suppressor function of TIM-3+ FoxP3+ regulatory T cells.
Gautron AS, Dominguez-Villar M, de Marcken M, Hafler DA
European journal of immunology 2014 Sep;44(9):2703-2711
European journal of immunology 2014 Sep;44(9):2703-2711
CD20+ B cell depletion alters T cell homing.
Kap YS, van Driel N, Laman JD, Tak PP, 't Hart BA
Journal of immunology (Baltimore, Md. : 1950) 2014 May 1;192(9):4242-53
Journal of immunology (Baltimore, Md. : 1950) 2014 May 1;192(9):4242-53
Superiority of rapamycin over tacrolimus in preserving nonhuman primate Treg half-life and phenotype after adoptive transfer.
Singh K, Stempora L, Harvey RD, Kirk AD, Larsen CP, Blazar BR, Kean LS
American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons 2014 Dec;14(12):2691-703
American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons 2014 Dec;14(12):2691-703
Regulatory T cells exhibit decreased proliferation but enhanced suppression after pulsing with sirolimus.
Singh K, Kozyr N, Stempora L, Kirk AD, Larsen CP, Blazar BR, Kean LS
American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons 2012 Jun;12(6):1441-57
American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons 2012 Jun;12(6):1441-57
Significant mobilization of both conventional and regulatory T cells with AMD3100.
Kean LS, Sen S, Onabajo O, Singh K, Robertson J, Stempora L, Bonifacino AC, Metzger ME, Promislow DE, Mattapallil JJ, Donahue RE
Blood 2011 Dec 15;118(25):6580-90
Blood 2011 Dec 15;118(25):6580-90
CD28 costimulation Impairs the efficacy of a redirected t-cell antitumor attack in the presence of regulatory t cells which can be overcome by preventing Lck activation.
Kofler DM, Chmielewski M, Rappl G, Hombach A, Riet T, Schmidt A, Hombach AA, Wendtner CM, Abken H
Molecular therapy : the journal of the American Society of Gene Therapy 2011 Apr;19(4):760-7
Molecular therapy : the journal of the American Society of Gene Therapy 2011 Apr;19(4):760-7
Fc receptor-like 3 protein expressed on IL-2 nonresponsive subset of human regulatory T cells.
Nagata S, Ise T, Pastan I
Journal of immunology (Baltimore, Md. : 1950) 2009 Jun 15;182(12):7518-26
Journal of immunology (Baltimore, Md. : 1950) 2009 Jun 15;182(12):7518-26
IL-7 enhances thymic human T cell development in "human immune system" Rag2-/-IL-2Rgammac-/- mice without affecting peripheral T cell homeostasis.
van Lent AU, Dontje W, Nagasawa M, Siamari R, Bakker AQ, Pouw SM, Maijoor KA, Weijer K, Cornelissen JJ, Blom B, Di Santo JP, Spits H, Legrand N
Journal of immunology (Baltimore, Md. : 1950) 2009 Dec 15;183(12):7645-55
Journal of immunology (Baltimore, Md. : 1950) 2009 Dec 15;183(12):7645-55
Allosuppressive donor CD4+CD25+ regulatory T cells detach from the graft and circulate in recipients after liver transplantation.
Demirkiran A, Bosma BM, Kok A, Baan CC, Metselaar HJ, Ijzermans JN, Tilanus HW, Kwekkeboom J, van der Laan LJ
Journal of immunology (Baltimore, Md. : 1950) 2007 May 15;178(10):6066-72
Journal of immunology (Baltimore, Md. : 1950) 2007 May 15;178(10):6066-72
Loss of IL-7 receptor alpha on CD4+ T cells defines terminally differentiated B cell-helping effector T cells in a B cell-rich lymphoid tissue.
Lim HW, Kim CH
Journal of immunology (Baltimore, Md. : 1950) 2007 Dec 1;179(11):7448-56
Journal of immunology (Baltimore, Md. : 1950) 2007 Dec 1;179(11):7448-56
Loss of IL-7 receptor alpha on CD4+ T cells defines terminally differentiated B cell-helping effector T cells in a B cell-rich lymphoid tissue.
Lim HW, Kim CH
Journal of immunology (Baltimore, Md. : 1950) 2007 Dec 1;179(11):7448-56
Journal of immunology (Baltimore, Md. : 1950) 2007 Dec 1;179(11):7448-56
CD127 expression inversely correlates with FoxP3 and suppressive function of human CD4+ T reg cells.
Liu W, Putnam AL, Xu-Yu Z, Szot GL, Lee MR, Zhu S, Gottlieb PA, Kapranov P, Gingeras TR, Fazekas de St Groth B, Clayberger C, Soper DM, Ziegler SF, Bluestone JA
The Journal of experimental medicine 2006 Jul 10;203(7):1701-11
The Journal of experimental medicine 2006 Jul 10;203(7):1701-11
Expression of interleukin (IL)-2 and IL-7 receptors discriminates between human regulatory and activated T cells.
Seddiki N, Santner-Nanan B, Martinson J, Zaunders J, Sasson S, Landay A, Solomon M, Selby W, Alexander SI, Nanan R, Kelleher A, Fazekas de St Groth B
The Journal of experimental medicine 2006 Jul 10;203(7):1693-700
The Journal of experimental medicine 2006 Jul 10;203(7):1693-700
The interleukin-7 receptor alpha chain transmits distinct signals for proliferation and differentiation during B lymphopoiesis.
Corcoran AE, Smart FM, Cowling RJ, Crompton T, Owen MJ, Venkitaraman AR
The EMBO journal 1996 Apr 15;15(8):1924-32
The EMBO journal 1996 Apr 15;15(8):1924-32
Cloning of the human and murine interleukin-7 receptors: demonstration of a soluble form and homology to a new receptor superfamily.
Goodwin RG, Friend D, Ziegler SF, Jerzy R, Falk BA, Gimpel S, Cosman D, Dower SK, March CJ, Namen AE
Cell 1990 Mar 23;60(6):941-51
Cell 1990 Mar 23;60(6):941-51
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- FIGURE 1 Identification of Tscm CD4 + CD45RA + CD45RO - CD62L + CCR7 + CD127 + CD27 + CD28 + CD95 + CD122 + T (Tscm) cell in human blood and lymph nodes. PBMCs were isolated from the blood of non-small cell lung cancer (NSCLC) patients (n=15) (NSCLC-PBMC) and healthy donors (n=11) (HD-PBMC); lymphocytes were isolated from the tumor-infiltrated lymph node of NSCLC patients who were collected blood at same time (n=7) (NSCLC-Ly); lymphocytes were isolated from the healthy lymph node of non lung cancer patients (n=7) (Normal-Ly), analyzed by flow cytometry. A, Representative flow cytometric analyses of CD4 + CD45RA + CD45RO - CD62L + CCR7 + CD127 + CD27 + CD28 + CD95 + CD122 + T cells, indicating Tscm cells. B, The frequency of the CD4 + Tscm cells in the HD-PBMC, NSCLC-PBMC, Normal-Ly, NSCLC-Ly. The events of CD4 + Tscm cells in the blood and lymph node from NSCLC patients and healthy donors, expressed as the mean+-SEM. C, The frequency of the CD8 + Tscm cells in the HD-PBMC, NSCLC-PBMC, Normal-Ly, NSCLC-Ly, expressed as the mean+-SEM. D, The events of Tscm of CD4 + and CD8 + cells in the blood and the lymph node from NSCLC patients and healthy donors. HD indicates healthy donors; IFN, interferon; PBMC, peripheral blood mononuclear cells; Tscm cell, stem cell-like memory T cell. * P
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- FIGURE 3 CD4 + CD45RA + CD45RO - CD95 - CD122 + CD127 + T cell population displays different phenotypes in human blood and lymph nodes. A, Flow cytometry plots showing IFN-gamma expression in the CD4 + CD45RA + /CD45RO - CD95 - CD122 + CD127 + T cells from the blood and lymph node of the non-small cell lung cancer (NSCLC) patients and healthy donors. B, The mean frequency (+-SEM) of the events of CD4 + CD45RA + /CD45RO - CD95 - CD122 + CD127 + T cells. C, The mean IFN-gamma production (+-SEM) of the CD4 + CD45RA + /CD45RO - CD95 - CD122 + CD127 + T cells. HD indicates healthy donors; IFN, interferon; PBMC, peripheral blood mononuclear cells; PMA, phorbol 12-myristate13-acetate. * P
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- Fig. 6 a Representative flow cytometry data, demonstrating the gating strategy used on PBMC for Treg identification and analysis. FSC-area vs. FSC-height was used for doublet discrimination. A ""dump"" channel was used to gate out dead cells (LIVE/DEAD fixable viability stain), CD14 + monocytes and CD19 + B cells, and lymphocytes were subsequently selected by FSC vs. SSC. CD4 + T cells were gated on the basis of CD4 vs. CD3 staining, then examined for expression of Ki67 and ICOS. Tregs were identified within the CD4 + T cell population as CD25 hi CD127 lo and Foxp3 + . b Longitudinal empirical data, linear mixed models and estimated means (left, centre and right-hand panels respectively) for Ki67+ and ICOS+ expression on CD4+ T cells, and the Treg proportion of CD4 cells ( P -values: *
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- Figure 6 In vivo knockdown of PITPNM3 in CD4 + T cells reverses immunosuppression and inhibits tumor progression in humanized mice. (A) Humanized mice bearing palpable MDA-MB-231 orthotopic xenografts were intraperitoneally injected daily for 14 days with PBS, 1 nmol CD4-aptamer-control siRNA (AsiC-con) or CD4-aptamer-siRNA targeting PITPNM3 (sequence in A , AsiC-PI) to assess the role of PITPNM3 in TI Tregs, and other T cells and tumor control. Experimental schematic is provided in Supplementary information, Figure S9A . (B) Representative immunoblots showing selective knockdown of PITPNM3 protein in PB CD4 + T cells, but not tumor xenografts ( n = 3). (C) PITPNM3 knockdown did not affect the distribution of human CD45 + hematopoietic cells, CD4 + and CD8 + T cells, and CD14 + monocytes in the peripheral blood of humanized mice. Representative flow plots are shown ( n = 3). (D , E) Effect of PITPNM3 knockdown on TI naive CD4 + , Tregs and CD8 + T cell numbers, and apoptosis by TUNEL assay in xenografts. D shows representative immunofluorescence microscopy images. Top row indicates CD4 + naive T cells by arrows; the second row indicates CD4 + CD45RO + Foxp3 - CD4 + memory T cells (yellow arrows) and Foxp3 + Tregs (white arrows). Scale bar, 50 mum. E shows number of cells of each subtype/high power field in eight mice ( ** P < 0.01, *** P < 0.001 compared to PBS group by Student's t -test). (F) Flow cytometry analysis of gated human CD3 + CD4 + cells isolated from xenogra
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- Figure 4. miR-21 Promotes Effector Cell Differentiation through AP-1 Activation (A) Naive CD4 + T cells were transfected with either scrambled control or miR-21-blocking locked nucleic acid (LNA21). After 48 hr, PDCD4 and b-actin expression were assessed by western blot. Representative blots and mean normalized intensities from four experiments are shown (mean +- SEM, two-tailed paired t test). (B) Naive CD4 + T cells were activated with anti-CD3 and anti-CD28 beads and transduced with a lentiviral vector expressing scrambled control or anti-miR-21. The representative histogram shows phosphorylated JNK in GFP + cells on day 3. The filled gray histogram represents unstimulated naive CD4 + T cells. Results from 7 experiments are expressed relative to the geometric mean fluorescence intensity (MFI) of controls (mean +- SEM, two-tailed paired t test). (C) Naive CD4 + T cells were activated and transduced as described in (B) and co-transfected with the AP-1 luciferase reporter plasmid and the Renilla luciferase control construct. On day 3, the activity of AP-1 firefly luciferase was measured and normalized to that of Renilla luciferase (n = 4, mean +- SEM, two-tailed paired t test). (D-F) Naive CD4 + T cells isolated from 20- to 35-year-old and 65- to 85-year-old individuals were activated with beads coated with anti-CD3 and anti-CD28 antibodies. (D) PDCD4 expression was quantified by RT-PCR on day 3 and day 5. Results are normalized to ACTB and presented relative to
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- Figure 6 Greater numbers of interleukin (IL)-7Ralpha high effector memory (EM) CD8 + T cells than IL-7Ralpha low EM CD8 + T cells in the skin. (A) Immunofluorescence staining (40x) of CD8 + T cells (green) from non-lesional (healthy, HC) or lesional atopic dermatitis skin. IL-7Ralpha + CD8 + T cells (upper panel) representing IL-7Ralpha high EM CD8 + T cells were stained with antibodies (Abs) to IL-7Ralpha (red); perforin + CD8 + T cells (lower panel) representing IL-7Ralpha low EM CD8 + T cells, were stained with Abs to perforin (red). The image of the box was magnified twice and placed to the right of each image. (B) A quantitative measurement of IL-7Ralpha + and perforin + CD8 + T cells (frequency and number per tissue) in panel (A) , representing IL-7Ralpha high and IL-7Ralpha low EM CD8 + T cells, respectively. Four images per slide were evaluated for quantification. Data are representative of four independent experiments. Bars represent the mean, and p -values were obtained using the Wilcoxon matched pairs test (for comparing frequency and number between the two CD8 + T cell subsets) and Mann-Whitney U test (for comparing IL-7Ralpha + /perforin + ratio between HC and dermatitis).