Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- GTX85865 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX85865, RRID:AB_10724319
- Product name
- IL7 Receptor alpha antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Human Colon, MALT (formalin-fixed, paraffin-embedded) stained with IL7R antibody (GTX85865) at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody LS-D1, alkaline phosphatase-streptavidin and chromogen.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- "Dot Blot of Rabbit anti-IL-7 Receptor antibody. Antigen: IL-7 Receptor and IL-7 Receptor pY449 forms of the immunizing peptide and THRB1 and THRB1 pY171 as controls. Load: 3.0, 0.3, 0.1, 0.05 ug as indicated. Primary antibody: IL-7 Receptor antibody at 1:400 for 45 min at 4°C. Secondary antibody: Dylight488 rabbit secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C."
- Validation comment
- Dot