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- Antibody Data
- Antigen structure
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- Western blot [2]
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- Product number
- NB110-85537 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB110-85537, RRID:AB_1201237
- Product name
- Rabbit Polyclonal IL-7R alpha/CD127 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This is specific for mouse IL-7 protein phosphorylated at Y449. A BLAST analysis was used to suggest cross-reactivity with IL-7 from human and rat sources based on 100% identity with the immunizing sequence.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.72 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: IL-7 R alpha/CD127 [p Tyr449] Antibody [NB110-85537] - Lane 1: thymocyte D1 cells. Lane 2: thymocyte D1 cells treated with IL7 (50 ng/ml). IP: with anti-Phosphotyrosine conjugated to Protein G agarose. Primary antibody: IL7pY449 antibody at 1:10000 for overnight at 4C. Secondary antibody: HRP-conjugated goat anti-rabbit antibody at 1:10,000 for 45 min at RT and ECL detection. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 55 kDa, 64 kDa for IL7pY449. Other band(s): unspecifics.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: IL-7 R alpha/CD127 [p Tyr449] Antibody [NB110-85537] - Load: thymocyte D1 cells treated with or without IL-7 (50 ng/ml) for 20 min. Primary antibody: IL7pY449 antibody at 1:1000 for overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 55 kDa, 64 kDa for IL7pY449. Other band(s): unspecifics.
