AF-306-PB

antibody from R&D Systems

Targeting: IL7R CD127, IL7RA

Western blot (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

AF-306-PB

Provider

R&D Systems

Product name

Human IL-7R alpha/CD127 Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects human IL-7 R alpha in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with recombinant rat IL-7 R alpha and less than 5% cross-reactivity with recombinant mouse IL-7 R alpha is observed.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Increased soluble IL-7 receptor concentrations associate with improved IL-7 therapy outcomes in SIV-infected ART-treated Rhesus macaques.

Steele AK, Carrasco-Medina L, Sodora DL, Crawley AM
PloS one 2017;12(12):e0188427

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Aberrant plasma IL-7 and soluble IL-7 receptor levels indicate impaired T-cell response to IL-7 in human tuberculosis.

Lundtoft C, Afum-Adjei Awuah A, Rimpler J, Harling K, Nausch N, Kohns M, Adankwah E, Lang F, Olbrich L, Mayatepek E, Owusu-Dabo E, Jacobsen M
PLoS pathogens 2017 Jun;13(6):e1006425

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DNA Methylation Regulates the Differential Expression of CX3CR1 on Human IL-7Rαlow and IL-7Rαhigh Effector Memory CD8+ T Cells with Distinct Migratory Capacities to the Fractalkine.

Shin MS, You S, Kang Y, Lee N, Yoo SA, Park K, Kang KS, Kim SH, Mohanty S, Shaw AC, Montgomery RR, Hwang D, Kang I
Journal of immunology (Baltimore, Md. : 1950) 2015 Sep 15;195(6):2861-9

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Isolation and characterization of human trophoblast side-population (SP) cells in primary villous cytotrophoblasts and HTR-8/SVneo cell line.

Takao T, Asanoma K, Kato K, Fukushima K, Tsunematsu R, Hirakawa T, Matsumura S, Seki H, Takeda S, Wake N
PloS one 2011;6(7):e21990

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Soluble HIV Tat protein removes the IL-7 receptor alpha-chain from the surface of resting CD8 T cells and targets it for degradation.

Faller EM, Sugden SM, McVey MJ, Kakal JA, MacPherson PA
Journal of immunology (Baltimore, Md. : 1950) 2010 Sep 1;185(5):2854-66

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A crucial role for Kupffer cell-derived galectin-9 in regulation of T cell immunity in hepatitis C infection.

Mengshol JA, Golden-Mason L, Arikawa T, Smith M, Niki T, McWilliams R, Randall JA, McMahan R, Zimmerman MA, Rangachari M, Dobrinskikh E, Busson P, Polyak SJ, Hirashima M, Rosen HR
PloS one 2010 Mar 4;5(3):e9504