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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AAR-043-50UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Adenylate Cyclase 3 (AC3) (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 µL
- Concentration
- 0.85 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat lung (lanes 1 and 4), rat brain (lanes 2 and 5) and hippocampus (lanes 3 and 6) lysates: - 1-3. Anti-Adenylate Cyclase 3 (AC3) (extracellular) Antibody (#AAR-043), (1:200).4-6. Anti-Adenylate Cyclase 3 (AC3) (extracellular) Antibody , preincubated with Adenylate Cyclase 3/AC3 (extracellular) Blocking Peptide (#BLP-AR043).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat lung (lanes 1 and 4), rat brain (lanes 2 and 5) and hippocampus (lanes 3 and 6) lysates: - 1-3. Anti-Adenylate Cyclase 3 (AC3) (extracellular) Antibody (#AAR-043), (1:200).4-6. Anti-Adenylate Cyclase 3 (AC3) (extracellular) Antibody , preincubated with Adenylate Cyclase 3/AC3 (extracellular) Blocking Peptide (#BLP-AR043).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of Adenylate cyclase type IIIin rat U-87 MG cells - Cell surface detection of Adenylate cyclase type III in intact living rat U-87 MG cells. A. Extracellular staining of cells using Anti-Adenylate Cyclase 3 (AC3) (extracellular) Antibody (#AAR-043), (1:50) followed by goat Anti-rabbit-AlexaFluor-594 secondary Antibody (red). B. Merge of A with live view of the cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of Adenylate cyclase type III in mouse hippocampal neurons - Immunohistochemical staining of mouse hippocampal sections using Anti-Adenylate Cyclase 3 (AC3) (extracellular) Antibody (#AAR-043), (1:400), (red). A. AC3 staining of neurons. B. Staining with mouse Anti-NeuN (green), as a neuronal marker on the same section. C. Merge of A and B demonstrates “primary cilia” - thin rod-like extensions from neurons in the pyramidal layer of the hippocampus.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cell surface detection ofADCY3by indirect flow cytometry in live intacthumanMEG-01 megakaryocyticleukemiacells: - (black line) cells. (red) Cells+ goat- Anti-rabbit-FITC. (green) Cells+ Anti-Adenylate Cyclase 3 (AC3) (extracellular) Antibody (#AAR-043), (2.5μg) + goat- Anti-rabbit-FITC.
