MA1-41245

antibody from Invitrogen Antibodies

Targeting: PMS2 H_DJ0042M02.9, HNPCC4, MLH4, PMSL2

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Recommended by provider)

Antibody Data

Product number

MA1-41245

Provider

Invitrogen Antibodies

Product name

PMS2 Monoclonal Antibody (163C1251)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

Suggested positive control: NIH-3T3.

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Antibody clone number

163C1251

Vial size

100 µg

Concentration

1.0 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PMS2 using a PMS2 monoclonal antibody (Product # MA1-41245) at 2 µg/mL against 10 µg of NIH 3T3 cell lysate.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PMS2 in 10 µg NIH 3T3 cell lysate. Samples were incubated in PMS2 monoclonal antibody (Product # MA1-41245) using a dilution of 2 µg/mL.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of PMS2 in formalin fixed paraffin-embedded (FFPE) human colon cancer. Samples were incubated in PMS2 monoclonal antibody (Product # MA1-41245) using a dilution of 5 µg/mL. Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Nuclear staining of PMS2 was observed. Staining was performed by Histowiz.