Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Chromatin Immunoprecipitation [1]
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Validation data
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- Product number
- PA5-29835 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PMS2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A431, HeLa, HepG2, human PMS2-transfected 293T. Predicted reactivity: Mouse (88%), Bovine (90%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PMS2 Polyclonal Antibody detects PMS2 protein by western blot analysis. A. 30 µg 293T whole cell extract. B. 30 µg whole cell extract of human PMS2-transfected 293T cells.7.5 % SDS-PAGE. PMS2 Polyclonal Antibody (Product # PA5-29835) dilution: 1:5,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PMS2 Polyclonal Antibody detects PMS2 protein by western blot analysis. A. 30 µg A431 whole cell extract. B. 30 µg HeLa whole cell extract. C. 30 µg HepG2 whole cell extract.7.5 % SDS-PAGE. PMS2 Polyclonal Antibody (Product # PA5-29835) dilution: 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-PMS2 Polyclonal Antibody (Product # PA5-29835) and a 95kDa band corresponding to PMS2 was observed in HeLa, MCF7, SH-SY5Y, LNCaP, Jurkat, PC-3 and not in Molt-4 and DU 145 which are reported to be negative. Two isoforms corresponding to ~ 51, ~62 kDa was observed across all the cell lines tested. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), MCF7 (Lane 2), SH-SY5Y (Lane 3), LNCaP (Lane 4), Jurkat (Lane 5), PC-3 (Lane 6), MOLT-4 (Lane 7) and DU 145 (Lane 8) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Chromatin Immunoprecipitation (ChIP) assay of endogenous PMS2 protein using Anti-PMS2 Antibody: ChIP was performed using Anti-PMS2 Rabbit Polyclonal Antibody (Product # PA5-29835, 5 µg) on sheared chromatin from methyl methanesulfonate treated SW480 cells using the MAGnify ChIP System kit (Product # 49-2024). Normal Rabbit IgG was used as a negative IP control. The purified DNA was analyzed by qPCR using primers binding to GAPDH transcriptional start site, GAPDH gene body (+2Kb), ACTB promoter, CDKN1A intron 1 and SATA satellite repeats. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.