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Western blot
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- NB100-56554 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-56554, RRID:AB_2237333
- Product name
- Mouse Monoclonal PMS2 Antibody
- Antibody type
- Monoclonal
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Supportive validation
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PMS2 Antibody (163C1251) [NB100-56554] - Analysis of PMS2 using this antibody at 2 ug/ml against 10 ug of NIH 3T3 cell lysate.
Supportive validation
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PMS2 Antibody (163C1251) [NB100-56554] - Analysis of formalin fixed paraffin-embedded (FFPE) human colon cancer using PMS2 antibody at 5 ug/ml on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Nuclear staining of PMS2 was observed. Staining was performed by Histowiz.
