Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 689702 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#689702, RRID:AB_2629789
- Product name
- Purified anti-SP4
- Antibody type
- Monoclonal
- Antigen
- Partial human SP4 recombinant protein (131-380 a.a.) expressed in E. coli.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- W15173A
- Vial size
- 100 µg
- Concentration
- 0.5 μ/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Total cell lysate from human OVCAR3 cells (lane 1) and MCF-7 cells (lane 2) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified anti-SP4 (clone W15173A) antibody. Proteins were visualized using an HRP goat anti-mouse IgG antibody and chemiluminescence detection. Purified anti-GAPDH (clone Poly6314) antibody was used as a loading control.
- Conjugate
- Unconjugated