Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [2]
- Other assay [1]
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- Product number
- PA5-59159 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SP3/SP4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: NQATGQQQII IDPSQGLVQL QNQPQQLELV TTQLAGNAWQ LVASTPPASK ENNVSQP Highest antigen sequence identity to the following orthologs: Mouse - 100%, Rat - 100%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of SP3/SP4 in human cell line U-251 MG shows positivity in cytoplasm & nucleus but excluded from the nucleoli. Samples were probed using a SP3/SP4 Polyclonal Antibody (Product # PA5-59159).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of SP3/SP4 in human cell line U-251 MG using a SP3/SP4 Polyclonal Antibody (Product # PA5-59159) shows localization to nucleoplasm and cytosol.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 6 Oestrogen-related receptors alpha ( ERR alpha) bound to SP 3 to increase the transcription of ABCB 1. The ERR alpha was immunoprecipitated by use of its specific antibody in MG -63/Dox (A) or HOS /Dox (B) and their parental cells, respectively. The bound SP 3 was measured by western blot analysis, IgG was used as the negative control; (C) MG -63/Dox cells were transfected with si- NC or si- SP 3 for 24 h; MG -63/Dox cells were transfected with si- NC or si- SP 3 for 12 h and then further treated with or without 1 mumol/L XCT -790 for 24 h, the mRNA (D) or protein (E) expression of P-gp was measured and quantitatively analyzed. Data are presented as means +- SD of three independent experiments. ** P < 0.01 compared with control