Antibody data
- Antibody Data
- Antigen structure
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- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- 12-1319-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD131 Monoclonal Antibody (1C1), PE, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The 1C1 monoclonal antibody reacts with the human CD131 molecule, also known as the common beta subunit (betaC). The common beta subunit associates with the specific alpha subunits of IL-3 receptor, IL-5 receptor and GM-CSF receptor to form high affinity receptors for these cytokines. These cytokine receptors are expressed by neutrophils, eosinophils, monocytes, endothelial cells, fibroblasts and hematopoietic progenitor cells and play a crucial role in growth/activation of eosinophils and in the inflammatory response. Applications Reported: This 1C1 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 1C1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Yellow dye
- Isotype
- IgG
- Antibody clone number
- 1C1
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD10 APC (Product # 17-0106-42) and Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (left) or Anti-Human CD131 PE (right). Cells in the granulocyte gate were used for analysis.
- Conjugate
- Yellow dye