Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Flow cytometry [1]
- Other assay [1]
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Validation data
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- Product number
- 12-1319-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD131 Monoclonal Antibody (1C1), PE, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The 1C1 monoclonal antibody reacts with the human CD131 molecule, also known as the common beta subunit (betaC). The common beta subunit associates with the specific alpha subunits of IL-3 receptor, IL-5 receptor and GM-CSF receptor to form high affinity receptors for these cytokines. These cytokine receptors are expressed by neutrophils, eosinophils, monocytes, endothelial cells, fibroblasts and hematopoietic progenitor cells and play a crucial role in growth/activation of eosinophils and in the inflammatory response. Applications Reported: This 1C1 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 1C1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Yellow dye
- Isotype
- IgG
- Antibody clone number
- 1C1
- Vial size
- 100 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Severe T cell hyporeactivity in ventilated COVID-19 patients correlates with prolonged virus persistence and poor outcomes.
Renner K, Schwittay T, Chaabane S, Gottschling J, Müller C, Tiefenböck C, Salewski JN, Winter F, Buchtler S, Balam S, Malfertheiner MV, Lubnow M, Lunz D, Graf B, Hitzenbichler F, Hanses F, Poeck H, Kreutz M, Orsó E, Burkhardt R, Niedermair T, Brochhausen C, Gessner A, Salzberger B, Mack M
Nature communications 2021 May 21;12(1):3006
Nature communications 2021 May 21;12(1):3006
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD10 APC (Product # 17-0106-42) and Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (left) or Anti-Human CD131 PE (right). Cells in the granulocyte gate were used for analysis.
- Conjugate
- Yellow dye
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 3 Plasma from COVID-19 patients has pronounced T-cell suppressive activity. Whole blood from a healthy donor was washed twice with medium to remove the plasma. Plasma from 10 healthy controls (healthy plasma; n = 10 biologically independent samples), 13 non-ventilated (non-vent. plasma; n = 13 biologically independent samples), and 15 mechanically ventilated (vent. plasma; n = 19 biologically independent samples) COVID-19 patients was added and samples were cultured with anti-CD3 for 24 h. In all, 23% of the non-ventilated and 33% of the ventilated COVID-19 patients were treated with steroids (marked in blue). None of the patients was treated with other immunosuppressive agents. Expression of indicated surface markers was quantified by flow cytometry on basophils, CD14 + monocytes and neutrophils. The absolute expression values of indicated markers are shown as mean fluorescence intensity (MFI) on basophils, CD14 + monocytes, and neutrophils. Each sample is represented by one dot, and the mean is marked in red. One-way ANOVA with Bonferroni multiple comparison test was used. Source data are provided as a Source Data file.
- Conjugate
- Yellow dye