AP12488c

antibody from Abgent

Targeting: MAPK14 CSBP1, CSBP2, CSPB1, Mxi2, p38, PRKM14, PRKM15

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP12488c

Provider

Abgent

Proper citation

Abgent Cat#AP12488c, RRID:AB_10837555

Product name

MAPK14 Antibody (Center T180/Y182)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

0.38 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

MAPK14 Antibody (Center T180/Y182) (Cat. #AP12488c) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the MAPK14 antibody detected the MAPK14 protein (arrow).

Primary Ab dilution

1:1000

Immunocytochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"Fluorescent confocal image of A549 cell stained with MAPK14 Antibody (Center T180/Y182)(Cat#AP12488c).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MAPK14 primary antibody (1:25, 1 h at 37!). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37!).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37!). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).MAPK14 immunoreactivity is localized to Vesicles significantly and Cytoplasm weakly."

Primary Ab dilution

1:10~50

Immunohistochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

MAPK14 Antibody (Center T180/Y182) (Cat. #AP12488c)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MAPK14 Antibody (Center T180/Y182) for immunohistochemistry. Clinical relevance has not been evaluated.

Primary Ab dilution

1:10~50