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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- AP7546b - Provider product page
- Provider
- Abcepta
- Proper citation
- Abgent Cat#AP7546b, RRID:AB_2297510
- Product name
- MAPK14 Antibody (C-term)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 0.33 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Submitted references Regulation of mechanical stress-induced MMP-13 and ADAMTS-5 expression by RUNX-2 transcriptional factor in SW1353 chondrocyte-like cells.
Activation of p38 MAPK and increased glucose transport in chronic hibernating swine myocardium.
Tetsunaga T, Nishida K, Furumatsu T, Naruse K, Hirohata S, Yoshida A, Saito T, Ozaki T
Osteoarthritis and cartilage / OARS, Osteoarthritis Research Society 2011 Feb;19(2):222-32
Osteoarthritis and cartilage / OARS, Osteoarthritis Research Society 2011 Feb;19(2):222-32
Activation of p38 MAPK and increased glucose transport in chronic hibernating swine myocardium.
McFalls EO, Hou M, Bache RJ, Best A, Marx D, Sikora J, Ward HB
American journal of physiology. Heart and circulatory physiology 2004 Sep;287(3):H1328-34
American journal of physiology. Heart and circulatory physiology 2004 Sep;287(3):H1328-34
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Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- The anti-P38 Pab (Cat. #AP7546b) is used in Western blot to detect P38 in Jurkat cell lysate.
- Primary Ab dilution
- 1:1000
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- Western blot analysis of P38 (arrow) using P38 Antibody (C-term) (Cat.#AP7546b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAPK14 gene (Lane 2) (Origene Technologies).
- Primary Ab dilution
- 1:1000
Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of MAPK14 Antibody (C-term)(Cat#AP7546b) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
- Primary Ab dilution
- 1:10~50
Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- MAPK14 Antibody (C-term) (Cat. #AP7546b) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Primary Ab dilution
- 1:10~50
