Explore
Validate
LearnPA5-34769
antibody from Invitrogen Antibodies
Targeting: MAPK14
CSBP1, CSBP2, CSPB1, Mxi2, p38, PRKM14, PRKM15
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-34769 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- p38 MAPK alpha Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2. Predicted reactivity: Mouse (99%), Rat (99%), Xenopus laevis (85%), Dog (95%), Sheep (96%), Chimpanzee (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.44 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MAPK14 using 30 µg of A) 293T (B) HepG2 and C) MOLT4 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a MAPK14 polyclonal antibody (Product # PA5-34769) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of p38 MAPK alpha was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a p38 MAPK alpha Polyclonal Antibody (Product # PA5-34769) at a dilution of 1:500 and a HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using p38 MAPK alpha Polyclonal Antibody (Product # PA5-34769). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with p38 MAPK alpha Polyclonal Antibody (Product # PA5-34769) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- p38 MAPK alpha Polyclonal Antibody detects p38 MAPK protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde for 10 min. Green: p38 MAPK protein stained by p38 MAPK alpha Polyclonal Antibody (Product # PA5-34769) diluted at 1:100. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of p38 MAPK alpha was performed in paraffin-embedded rat brain tissue using p38 MAPK alpha Polyclonal Antibody (Product # PA5-34769) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- p38 antibody [N1C3-2] detects p38 protein at nucleus on human lung adenocarcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung adenocarcinoma. P38 antibody [N1C3-2] (Product # PA5-34769) diluted at 1:250. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
