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LearnM00176-1
antibody from Boster Biological Technology
Targeting: MAPK14
CSBP1, CSBP2, CSPB1, Mxi2, p38, PRKM14, PRKM15
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [9]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- M00176-1 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-p38 MAPK MAPK14 Rabbit Monoclonal Antibody
- Antibody type
- Monoclonal
- Description
- Monoclonal antibody for P38/MAPK14 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: IF, IHC, ICC, WB. Reactive species: Human, Mouse, Rat P38/MAPK14 information: Molecular Weight: 41293 MW; Subcellular Localization: Cytoplasm . Nucleus ; Tissue Specificity: Brain, heart, placenta, pancreas and skeletal muscle. Expressed to a lesser extent in lung, liver and kidney.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Antibody clone number
- EGC-13
- Vial size
- 100ug/vial
- Concentration
- 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
- Storage
- At -20°C for one year. Avoid repeated freezing and thawing.
Submitted references Dehydrocostus lactone (DHC) promotes osteoblastic differentiation and mineralization through p38/RUNX-2 signaling.
Active fractions from Jingfang Baidu Powder alleviate Klebsiella-induced Pneumonia by inhibiting TLR4/Myd88-ERK signaling pathway.
Seed oil of Rosa roxburghii Tratt against non-alcoholic fatty liver disease in vivo and in vitro through PPARα/PGC-1α-mediated mitochondrial oxidative metabolism.
Total Panax notoginseng saponin inhibits balloon injury-induced neointimal hyperplasia in rat carotid artery models by suppressing pERK/p38 MAPK pathways.
Tenacissoside H exerts an anti-inflammatory effect by regulating the nf-κb and p38 pathways in zebrafish.
Deoxynivalenol induces toxicity and apoptosis in piglet hippocampal nerve cells via the MAPK signaling pathway.
Vasopressin decreases neuronal apoptosis during cardiopulmonary resuscitation.
Cucurbitacin B inhibits growth and induces apoptosis through the JAK2/STAT3 and MAPK pathways in SH‑SY5Y human neuroblastoma cells.
Panax notoginseng saponins promote osteogenic differentiation of bone marrow stromal cells through the ERK and P38 MAPK signaling pathways.
Wu S, Bai X, Cai L, Ke Q, Zhang X
Journal of biochemical and molecular toxicology 2024 Jan;38(1):e23601
Journal of biochemical and molecular toxicology 2024 Jan;38(1):e23601
Active fractions from Jingfang Baidu Powder alleviate Klebsiella-induced Pneumonia by inhibiting TLR4/Myd88-ERK signaling pathway.
Sun C, Xu Y, Xu G, Ji X, Jiang P, He Y
Journal of ethnopharmacology 2024 Aug 10;330:118067
Journal of ethnopharmacology 2024 Aug 10;330:118067
Seed oil of Rosa roxburghii Tratt against non-alcoholic fatty liver disease in vivo and in vitro through PPARα/PGC-1α-mediated mitochondrial oxidative metabolism.
Ni HY, Yu L, Zhao XL, Wang LT, Zhao CJ, Huang H, Zhu HL, Efferth T, Gu CB, Fu YJ
Phytomedicine : international journal of phytotherapy and phytopharmacology 2022 Apr;98:153919
Phytomedicine : international journal of phytotherapy and phytopharmacology 2022 Apr;98:153919
Total Panax notoginseng saponin inhibits balloon injury-induced neointimal hyperplasia in rat carotid artery models by suppressing pERK/p38 MAPK pathways.
Yang Z, Zhang H, An M, Bian M, Song M, Guo X, Liu Q, Qiu M
Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas 2020;53(1):e9085
Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas 2020;53(1):e9085
Tenacissoside H exerts an anti-inflammatory effect by regulating the nf-κb and p38 pathways in zebrafish.
Li JJ, Zhang Y, Han LW, Tian QP, He QX, Wang XM, Sun C, Han J, Liu KC
Fish & shellfish immunology 2018 Dec;83:205-212
Fish & shellfish immunology 2018 Dec;83:205-212
Deoxynivalenol induces toxicity and apoptosis in piglet hippocampal nerve cells via the MAPK signaling pathway.
Wang X, Fan M, Chu X, Zhang Y, Rahman SU, Jiang Y, Chen X, Zhu D, Feng S, Li Y, Wu J
Toxicon : official journal of the International Society on Toxinology 2018 Dec 1;155:1-8
Toxicon : official journal of the International Society on Toxinology 2018 Dec 1;155:1-8
Vasopressin decreases neuronal apoptosis during cardiopulmonary resuscitation.
Ma C, Zhu Z, Wang X, Zhao G, Liu X, Li R
Neural regeneration research 2014 Mar 15;9(6):622-9
Neural regeneration research 2014 Mar 15;9(6):622-9
Cucurbitacin B inhibits growth and induces apoptosis through the JAK2/STAT3 and MAPK pathways in SH‑SY5Y human neuroblastoma cells.
Zheng Q, Liu Y, Liu W, Ma F, Zhou Y, Chen M, Chang J, Wang Y, Yang G, He G
Molecular medicine reports 2014 Jul;10(1):89-94
Molecular medicine reports 2014 Jul;10(1):89-94
Panax notoginseng saponins promote osteogenic differentiation of bone marrow stromal cells through the ERK and P38 MAPK signaling pathways.
Li XD, Liu ZY, Chang B, Liu DX, Chen B, Guo C, Wang YG, Xu JK, Huang DY, Du SX
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2011;28(2):367-76
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2011;28(2):367-76
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of p38 MAPK expression in Jurkat cell lysate (M00176-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAPK14 monoclonal antibody (Catalog # M00176-1) overnight at 4
- Additional image
