Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Other assay [1]
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- Product number
- PA5-81518 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-c-MAF Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%). This antibody has specificity for Human c-Maf/MAF.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- Lot Dependent
- Storage
- Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of c-MAF in Lane A: PC12 Whole Cell Lysate (30 µg). Samples were probed using a c-MAF Polyclonal Antibody (Product # PA5-81518) at a 1:500 dilution, followed by a Goat Anti-Rabbit IgG (H+L), Dylight 800 Secondary Antibody at a 1:10000 dilution. Western blot was performed under reducing conditions. Predicted band size:42 kDa. Observed band size:47 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- c-MAF was immunoprecipitated using: Lane A: 0.5 mg 293T Whole Cell Lysate, 1 µL of c-MAF Polyclonal Antibody and 15 µL of 50% Protein G agarose. Primary Antibody: c-MAF Polyclonal Antibody (Product # PA5-81518), at 1:500 dilution. Secondary Antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Immunoprecipitation was performed under reducing conditions. Predicted band size: 41 kDa. Observed band size: 41 kDa.