A700-045

antibody from Invitrogen Antibodies

Targeting: MAF c-MAF

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A700-045

Provider

Invitrogen Antibodies

Product name

c-Maf Recombinant Rabbit Monoclonal Antibody (BLR045F)

Antibody type

Monoclonal

Antigen

Other

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Antibody clone number

BLR045F

Vial size

100 µL

Concentration

1000 µg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human c-Maf by western blot. Samples: Whole cell lysate (50 µg) from RPMI-8226, Hep-G2, 786-O, and Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-c-Maf recombinant monoclonal antibody [BL-664A-5F2] (Product # A700-045 lot 3) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 30 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human c-Maf by western blot of HEK293T transfected with myc tagged human or mouse MafA, MafB, or c-Maf. Antibody: Rabbit anti-c-Maf recombinant monoclonal antibody [BLR045R] (Product # A700-045 lot 3) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Myc Tag recombinant monoclonal antibody [BLRE01G] (Product # A191-101A).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human c-MAF by immunocytochemistry.Sample: FFPE section of RPMI-8226 cells.Antibody: Rabbit anti-c-MAF recombinant monoclonal antibody [BLR045F] (Product # A700-045; lot 3) used at 1:125.Secondary: HRP-conjugated goat anti-rabbit IgG (Product # A120-501P). Substrate: DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human c-MAF by immunocytochemistry.Sample: FFPE section of RPMI-8226 cells.Antibody: Rabbit anti-c-MAF recombinant monoclonal antibody [BLR045F] (Product # A700-045; lot 3) used at 1:125.Secondary: HRP-conjugated goat anti-rabbit IgG (Product # A120-501P). Substrate: DAB.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human c-MAF by immunohistochemistry.Sample: FFPE section of human tonsil.Antibody: Rabbit anti-c-MAF recombinant monoclonal antibody [BLR045F] (Product # A700-045 lot 3) used at 1:125.Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of mouse c-MAF by immunohistochemistry.Sample: FFPE section of mouse renal cell carcinoma.Antibody: Rabbit anti-c-MAF recombinant monoclonal antibody [BLR045F] (Product # A700-045 lot 3) used at 1:125.Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human c-Maf by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-c-Maf recombinant monoclonal antibody [BL-664A-5F2] (Product # A700-045 lot 3) used for IP at 6 µL per reaction. c-Maf was also immunoprecipitated by a previous lot of this antibody (lot 2). For blotting immunoprecipitated c-Maf (Product # A700-045) was used at 1:1000. Chemiluminescence with an exposure time of 10 seconds.