LS-C186541

antibody from LSBio

Targeting: MAPKAPK2 MK2

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C186541

Provider

LSBio

Product name

MAPKAPK2 / MAPKAP Kinase 2 Antibody (aa44-55) LS-C186541

Antibody type

Polyclonal

Description

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Reactivity

Human, Mouse, Simian

Host

Goat

Storage

Aliquot and store at -20°C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

MAPKAPK2 antibody EB11929 and (1.5 ug) immunoprecipitations from lysates of MK2/MK3 double knockout MEFs, with (third and fifth lanes) and without (fourth and sixth lanes) rescued MK2 expression through retroviral transduction. The corresponding lysates (first and second lane resp.) were analyzed in parallel in this Western blot labeled with rabbit anti-MK2.

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

MAPKAPK2 antibody HEK293 overexpressing Mouse MK2 fused to GFP or overexpressing MK5 fused to GFP and probed with EB11929 (0.5 ug/ml) in the left panel and with (0.5 ug/ml) in the right panel.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

MAPKAPK2 antibody HEK293 overexpressing Mouse MK2 fused to GFP or overexpressing MK5 fused to GFP and probed with EB11929 (0.5 ug/ml) in the left panel and with (0.5 ug/ml) in the right panel.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

MAPKAPK2 antibody HEK293 overexpressing Mouse MK2 fused to GFP or overexpressing MK5 fused to GFP and probed with EB11929 (0.5 ug/ml) in the left panel and with (0.5 ug/ml) in the right panel.