LS-B10396

antibody from LSBio

Targeting: MAPKAPK2 MK2

Western blot (Enhanced validation)

ELISA (Recommended by provider)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

LS-B10396

Provider

LSBio

Product name

IHC-plus™ MAPKAPK2 / MAPKAP Kinase 2 Antibody (aa339-351) LS-B10396

Antibody type

Polyclonal

Description

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Reactivity

Human, Mouse, Bovine, Canine, Guinea Pig, Hamster, Horse, Porcine, Rabbit

Host

Goat

Storage

Aliquot and store at -20°C. Avoid freeze-thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

MAPKAPK2 / MAPKAP Kinase 2 antibody (0.5µg/ml) staining of MEF lysates (35µg protein in RIPA buffer), from double KO mice in second and third lanes and rescued by introduction of MK2 gene in second lane. Primary incubation was 2 hour. Detected by chemiluminescence.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

MAPKAPK2 antibody and EB11930 (1.5 ug) immunoprecipitations from lysates of MK2/MK3 double knockout MEFs, with (third and fifth lanes) and without (fourth and sixth lanes) rescued MK2 expression through retroviral transduction. The corresponding lysates (first and second lane resp.) were analyzed in parallel in this Western blot labeled with rabbit anti-MK2.

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

MAPKAPK2 antibody HEK293 overexpressing Mouse MK2 fused to GFP or overexpressing MK5 fused to GFP and probed with (0.5 ug/ml) in the left panel and with EB11930 (0.5 ug/ml) in the right panel.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

MAPKAPK2 antibody HEK293 overexpressing Mouse MK2 fused to GFP or overexpressing MK5 fused to GFP and probed with (0.5 ug/ml) in the left panel and with EB11930 (0.5 ug/ml) in the right panel.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

MAPKAPK2 antibody HEK293 overexpressing Mouse MK2 fused to GFP or overexpressing MK5 fused to GFP and probed with (0.5 ug/ml) in the left panel and with EB11930 (0.5 ug/ml) in the right panel.