AF3925a

antibody from Abcepta

Targeting: MAPKAPK2 MK2

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

AF3925a

Provider

Abcepta

Product name

"MK2 / MAPKAPK2 Antibody (internal region, near C-Term)"

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide-affinity purified goat antibody

Reactivity

Rat, Porcine

Host

Goat

Conjugate

Unconjugated

Isotype

IgG

Vial size

0.1mg

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -1509°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

"AF3925a (0.5 µg/ml) staining of MEF lysates (35 µg protein in RIPA buffer), from double KO mice in second and third lanes and rescued by introduction of MK2 gene in second lane. Primary incubation was 2 hour. Detected by chemiluminescence."

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

HEK293 overexpressing Mouse MK2 fused to GFP or overexpressing MK5 fused to GFP and probed with AF3925a (0.5ug/ml) in the left panel and with EB11930 (0.5ug/ml) in the right panel.

Immunoprecipitation

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

HEK293 overexpressing Mouse MK2 fused to GFP or overexpressing MK5 fused to GFP and probed with AF3925a (0.5ug/ml) in the left panel and with EB11930 (0.5ug/ml) in the right panel.