AM32520PU-N

antibody from Acris Antibodies GmbH

Targeting: MAPKAPK2 MK2

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AM32520PU-N

Provider

Acris Antibodies GmbH

Product name

anti MAPKAP Kinase-2 pThr334

Antibody type

Monoclonal

Antigen

Synthetic peptide containing the sequence and surrounding amino acids phosphorylated Thr334 of Human, Mouse, Rat, Bovine and Chicken MAPKAP Kinase 2. Immunizing peptide shared 66% homology with phospho-MAPKAP-K3 at Thr313

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

9F10.2

Vial size

0.1 mg

Concentration

1.0 mg/ml

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western Blot Analysis: PC12 cell lysates were resolved by electrophoresis, transferred to PVDF, and probed with were either unblocked (Lane 1), pre-blocked with phosphorylated peptide (Lane 2), or pre-blocked with unphosphorylated peptide (Lane 3) Anti-phospho-MAPKAPK2 (Thr334), clone 9F10.2 (1/1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates phosphorylated MAPKAPK2 (~50 kDa)

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western Blot Analysis: PC12 cell lysate was resolved by SDS-PAGE, transferred to PVDF, and probed with anti-phospho-MAPKAPK2 (Thr334), clone 9F10.2 (1/1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates phosphorylated MAPKAPK2 (~50 kDa).

Immunocytochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunofluorescence Confocal Microscopy Analysis: NIH/3T3 cells were fixed, permeabilized, and stained using Anti-phospho-MAPKAPK2 (Thr334), clone 9F10.2 (Red). Cells were costained for actin and nuclear staining with Phalloidin, AlexaFluor® 488 conjugate (Green) and DAPI (Blue), respectively.