AP32810PU-N

antibody from Acris Antibodies GmbH

Targeting: MAPKAPK2 MK2

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

AP32810PU-N

Provider

Acris Antibodies GmbH

Product name

anti MAPKAP Kinase-2

Antibody type

Polyclonal

Antigen

Peptide with sequence from the internal region (near C-Terminus) of the protein sequence according to NP_004750.1; NP_116584.2.

Reactivity

Human, Mouse, Rat, Bovine, Canine

Host

Goat

Vial size

0.1 mg

Concentration

0.5 mg/ml

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

HEK293 overexpressing Mouse MK2 fused to GFP or overexpressing MK5 fused to GFP and probed with AP32810PU-N (0.5 µg/ml) in the left panel and with AP32811PU-N (0.5 µg/ml) in the right panel.

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

AP32810PU-N  (0.5 µg/ml) staining of MEF lysates (35µg protein in RIPA buffer), from double KO mice in second and third lanes and rescued by introduction of MK2 gene in second lane. Primary incubation was 2 hour. Detected by chemiluminescence.

Immunoprecipitation

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

AP32810PU-N and AP32811PU-N (1.5 µg) immunoprecipitations from lysates of MK2/MK3 double knockout MEFs, with (third and fifth lanes) and without (fourth and sixth lanes) rescued MK2 expression through retroviral transduction. The corresponding lysates (first and second lane resp.) were analyzed in parallel in this Western blot labelled with rabbit anti-MK2.