Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
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Validation data
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- Product number
- AP32810PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Product name
- anti MAPKAP Kinase-2
- Antibody type
- Polyclonal
- Antigen
- Peptide with sequence from the internal region (near C-Terminus) of the protein sequence according to NP_004750.1; NP_116584.2.
- Reactivity
- Human, Mouse, Rat, Bovine, Canine
- Host
- Goat
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- HEK293 overexpressing Mouse MK2 fused to GFP or overexpressing MK5 fused to GFP and probed with AP32810PU-N (0.5 µg/ml) in the left panel and with AP32811PU-N (0.5 µg/ml) in the right panel.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- AP32810PU-N  (0.5 µg/ml) staining of MEF lysates (35µg protein in RIPA buffer), from double KO mice in second and third lanes and rescued by introduction of MK2 gene in second lane. Primary incubation was 2 hour. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- AP32810PU-N and AP32811PU-N (1.5 µg) immunoprecipitations from lysates of MK2/MK3 double knockout MEFs, with (third and fifth lanes) and without (fourth and sixth lanes) rescued MK2 expression through retroviral transduction. The corresponding lysates (first and second lane resp.) were analyzed in parallel in this Western blot labelled with rabbit anti-MK2.