Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- AP32811PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Product name
- anti MAPKAP Kinase-2 (44-55)
- Antibody type
- Polyclonal
- Antigen
- Peptide with sequence (44-55) from the internal region of the protein sequence according to NP_004750.1; NP_116584.2.
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- HEK293 overexpressing Mouse MK2 fused to GFP or overexpressing MK5 fused to GFP and probed with AP32810PUN (0.5 μg/ml) in the left panel and with AP32811PU-N (0.5 μg/ml) in the right panel.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- AP32810PU-N and AP32811PU-N (1.5 μg) immunoprecipitations from lysates of MK2/MK3 double knockout MEFs, with (third and fifth lanes) and without (fourth and sixth lanes) rescued MK2 expression through retroviral transduction. The corresponding lysates (first and second lane resp.) were analyzed in parallel in this Western blot labelled with Rabbit anti-MK2.