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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
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- Product number
- R35113 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- MAPKAPK2 Antibody
- Antibody type
- Polyclonal
- Description
- This highly specific MAPKAPK2 antibody is suitable for use in Western blot/ELISA/Immunoprecipitation applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5 mg/ml in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide
- Storage
- Aliquot and store the MAPKAPK2 antibody at -20oC.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot of HEK293 lysate overexpressing mouse MK2/MAPKAPK2-GFP or overexpressing MK5-GFP and probed with MAPKAPK2 antibody (0.5ug/ml, catalog # R36061) in the left panel and with MAPKAPK2 antibody (0.5ug/ml, cat # R35113) in the right panel.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunoprecipitation: MAPKAPK2 antibody (cat # R36061 and cat # R35113 at 1.5ug) precipitates from lysates of MK2/MK3 double knockout MEFs, with (third and fifth lanes) and without (fourth and sixth lanes) rescued MK2 expression through retroviral transduction.
