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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunoprecipitation [1]
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- Product number
- R36061 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- MAPKAPK2 Antibody
- Antibody type
- Polyclonal
- Description
- This highly specific MAPKAPK2 antibody is suitable for use in Western blot/Immunoprecipitation/ELISA applications with human and mouse samples.
- Reactivity
- Human, Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5 mg/ml in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide
- Storage
- Aliquot and store the MAPKAPK2 antibody at -20oC.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of mouse embryonic fibroblasat lysates from double KO mice (lanes 2 & 3) and rescued by introduction of MK2 gene in second lane. MK2 / MAPKAPK2 antibody was used at 0.5ug/ml. Predicted molecular weight: ~45kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot of HEK293 lysate overexpressing mouse MK2-GFP or MK5-GF probed with MAPKAPK2 antibody at 0.5ug/ml: cat # R36061 on left and cat # R35113 on right. Predicted molecular weight of MAPKAPK2+GFP: ~72kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunoprecipitation: MAPKAPK2 antibody (cat # R36061 and cat # R35113, 1.5ug used) precipitates from lysates of MK2/MK3 double knockout MEFs, with (third and fifth lanes) and without (fourth and sixth lanes) rescued MK2 expression through retroviral transduction.
