Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- Ab115490 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- Recombinant MK2 Antibody
- Antibody type
- Recombinant
- Description
- Rabbit anti Human MK2 Antibody, Recombinant, could be used for WB and so on.Application:WB: 1/500-1/1000Protein FunctionIts physiological substrate seems to be the small heat shock protein (HSP27/HSP25). In vitro can phosphorylate glycogen synthase at 'Ser-7' and tyrosine hydroxylase (on 'Ser-19' and 'Ser-40'). This kinase phosphorylates Ser in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue (By similarity). Mediates both ERK and p38 MAPK/MAPK14 dependent neutrophil responses. Participates in TNF alpha-stimulated exocytosis of secretory vesicles in neutrophils. Plays a role in phagocytosis-induced respiratory burst activity.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
AA 377-391
- Vial size
- 100_l,10_l,1ml,50_l
- Concentration
- 0,8 mg/ml
- Storage
- Store at 4¡C short term (1-2 weeks). Store at -20¡C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
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- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant MK2 Antibody (Ab115490) - Western Blot All lanes: Recombinant MK2 Antibody (Ab115490) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 46 kDa Observed band size: 45, 50 kDa Exposure time: 1.1 s