PA5-17729

antibody from Invitrogen Antibodies

Targeting: MAPKAPK2 MK2

Antibody data

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Validation data

MAPKAPK2 protein structure - PA5-17729 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of MAP kinase-activated protein kinase 2 was achieved by transfecting HeLa with MAP kinase-activated protein kinase 2 specific siRNAs (Silencer® select Product # s570, s571). Western blot analysis (Fig. a) was performed using Whole cell extracts from the MAP kinase-activated protein kinase 2 knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with MAPKAPK2 Polyclonal Antibody (Product # PA5-17729, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to MAP kinase-activated protein kinase 2.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-MAPKAPK2 Polyclonal Antibody (Product # PA5-17729) and a 45 kDa band corresponding to MAP kinase-activated protein kinase 2 was observed across cell lines tested. Whole cell extracts (30 µg lysate) of MCF7 (Lane 1), HeLa (Lane 2), Reh (Lane 3), A549 (Lane 4), A-431 (Lane 5), Jurkat (Lane 6), HEK-293 (Lane 7) and NIH:OVCAR-3 (Lane 8) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Reagent Kit (Product # WP20005).