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- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- ABIN1504200 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Mitogen-Activated Protein Kinase-Activated Protein Kinase 2 (MAPKAPK2) antibody
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Protein G Chromatography
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- '9F10-2
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/mL
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western Blot Analysis: PC12 cell lysate was resolved by SDS-PAGE, transferred to PVDF, and probed with anti-phospho-MAPKAPK2 (Thr334), clone 9F10.2 (1/1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates phosphorylated MAPKAPK2 (~50 kDa).
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western Blot Analysis: PC12 cell lysates were resolved by electrophoresis, transferred to PVDF, and probed with were either unblocked (Lane 1), pre-blocked with phosphorylated peptide (Lane 2), or pre-blocked with unphosphorylated peptide (Lane 3) Anti-phospho-MAPKAPK2 (Thr334), clone 9F10.2 (1/1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates phosphorylated MAPKAPK2 (~50 kDa)
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Immunofluorescence Confocal Microscopy Analysis: NIH/3T3 cells were fixed, permeabilized, and stained using Anti-phospho-MAPKAPK2 (Thr334), clone 9F10.2 (Red). Cells were costained for actin and nuclear staining with Phalloidin, AlexaFluor® 488 conjugate (Green) and DAPI (Blue), respectively.
