M550B

antibody from Invitrogen Antibodies

Targeting: IL5 EDF, IL-5, TRF

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

M550B

Provider

Invitrogen Antibodies

Product name

IL-5 Monoclonal Antibody (39D10), Biotin

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

M550B targets IL-5 in ELISA, and WB applications and shows reactivity with Human samples.

Conjugate

Biotin

Antibody clone number

39D10

Concentration

0.5 mg/mL

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Sandwich ELISA analysis of human IL-5 was performed by coating wells of a 96-well plate with 110 µL of a human IL-5 monoclonal antibody (Product # M551) in duplicate at 1, 2, 4, 5, 7, and 9 µg/mL for at least 12 hours. The plate was blocked with a proprietary blocking solution for 1-4 hours at room temperature, and 50 µL of mouse IL-5 recombinant protein (Product # SMIL5) was added in replicates of twelve at 400, 160, 64, 25.6, 10.24, and 0pg/mL for 1 hour at room temperature. The plate was washed and incubated 50 µL of a biotinylated IL-5 monoclonal antibody (Product # M550B) was added to all test wells at 0.5 µg/mL for 1 hour at room temperature. The plate was washed, then incubated with 100 µL per well of Streptavidin-HRP (Product # N504) at a 1:10,000 dilution for 30 minutes at room temperature. Detection was performed with 100 µL per well of Ultra TMB substrate (Product # 34028) for 30 minutes at room temperature in the dark. The plate was then stopped with 0.16M sulfuric acid. Absorbances were read on a spectrophotometer at 450-550 nm.

Conjugate

Biotin