Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- 504311 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#504311, RRID:AB_2563161
- Product name
- Brilliant Violet 421™ anti-mouse/human IL-5
- Antibody type
- Monoclonal
- Antigen
- Partially-purified T cell clone supernatant
- Description
- IL-5 is a homodimeric, disulphide-linked protein produced by T-cells. Monomeric human IL-5 is a 126 amino acid protein with a reported molecular weight of 26 kD for the homodimeric protein. Mouse and human IL-5 are approximately 70% identical. IL-5 has been shown to promote the growth of immature hematopoietic BFU-E progenitors, stimulate the activation and differentiation of eosinophils, and promote the generation of cytotoxic lymphocytes. Mouse IL-5 induces the differentiation and proliferation of pre-activated B-cells and stimulates the production and secretion of IgM and IgA by B-cells stimulated with bacterial endotoxin. The TRFK5 antibody can neutralize the bioactivity of natural or recombinant IL-5.
- Reactivity
- Human, Mouse, Guinea Pig
- Host
- Rat
- Conjugate
- Blue dye
- Antibody clone number
- TRFK5
- Vial size
- 50 µg
- Concentration
- 0.2 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
- Main image
- Experimental details
- PMA+ionomycin (6 hours)-stimulated Th2 polarized BALB/c splenocytes were stained with CD3 APC, and then intracellularly stained with IL-5 (clone TRFK5) Brilliant Violet 421™ or rat IgG1 Brilliant Violet BV421™ isotype control (bottom).
- Conjugate
- Blue dye