AB-205-NA

antibody from Novus Biologicals

Targeting: IL5 EDF, IL-5, TRF

Western blot (Data presented on provider website)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

AB-205-NA

Provider

Novus Biologicals

Product name

Goat Polyclonal IL-5 Antibody

Antibody type

Polyclonal

Description

Protein A or G purified. Detects human IL-5 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant mouse IL-5 is observed and 15% cross-reactivity with recombinant rat IL-5 is observed. Neutralizes the biological activity of recombinant human IL-5 and will also neutralize the biological activity of recombinant mouse IL-5, although four times the amount of IgG is required.

Reactivity

Human

Host

Goat

Isotype

IgG

Vial size

1 mg

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Inhibition of interleukin-5 induced false positive anti-drug antibody responses against mepolizumab through the use of a competitive blocking antibody.

Liao K, Meyer E, Lee TN, Loercher A, Sikkema D
Journal of immunological methods 2017 Feb;441:15-23

Blocking/Neutralizing

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Cell Proliferation Induced by IL-5 and Neutralization by Human IL-5 Antibody. Recombinant Human IL-5 (Catalog # 205-IL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-5 (1.25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-5 Polyclonal Antibody (Catalog # AB-205-NA). The ND₅₀ is typically 8-10 µg/mL.